PXD045835

PXD045835 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas aeruginosa Heme Metabolites Biliverdin IX and IX are Integral to Lifestyle Adaptations Associated with Chronic Infection
Description	Pseudomonas aeruginosa is a versatile opportunistic pathogen requiring iron for its survival and virulence within the host. The ability to switch to heme as an iron source provides an advantage in chronic infection. We have recently shown the extracellular heme metabolites biliverdin IX (BVIX) and/or BVIX positively regulate the heme dependent cell surface signaling cascade. We further investigated the role of BVIX and BVIX in cell signaling utilizing allelic strains lacking a functional HemO (hemOin), or one reengineered to produce BVIX (hemO). Compared to PAO1 both strains show a heme dependent growth defect, decreased swarming and twitching and less robust biofilm formation. Interestingly, the motility and biofilm defects were partially rescued on addition of exogenous BVIX and BVIX. Utilizing LC-MS/MS we performed a comparative proteomics and metabolomics analysis of PAO1 versus the allelic strains in shaking and static conditions. In shaking conditions, the hemO allelic strains showed a significant increase in proteins involved in quorum sensing (QS), phenazine production and chemotaxis.Metabolite profiling further revealed increased levels of PQS and phenazine metabolites. In static conditions we observed a significant repression of chemosensory pathways and Type IV pili (TFP) biogenesis proteins as well as several phosphodiesterases associated with biofilm dispersal. We propose BVIX metabolites function as signaling and chemotactic molecules integrating heme utilization as an iron source into the adaptation of P. aeruginosa from a planktonic to sessile lifestyle.
HostingRepository	PRIDE
AnnounceDate	2024-01-19
AnnouncementXML	Submission_2024-01-19_13:27:05.641.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehari Weldemariam
SpeciesList	scientific name: Pseudomonas aeruginosa PABL056; NCBI TaxID: 1214916;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 18:25:26	ID requested
⏵ 1	2024-01-19 13:27:06	announced

10.1128/MBIO.02763-23;

submitter keyword: proteomics, metabolomics, Type IV pili and chemotaxis, heme, biliverdin,Pseudomonas aeruginosa

Kane Maureen
contact affiliation	Department of Pharmaceutical Sciences School of Pharmacy University of Maryland Baltimore
contact email	mkane@rx.umaryland.edu
lab head
Mehari Weldemariam
contact affiliation	University of Maryland Baltimore
contact email	mweldemariam@rx.umaryland.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045835

PRIDE project URI

• PRIDE
  1. PXD045835
     1. Label: PRIDE project
     2. Name: Pseudomonas aeruginosa Heme Metabolites Biliverdin IX and IX are Integral to Lifestyle Adaptations Associated with Chronic Infection