PXD045826

PXD045826 is an original dataset announced via ProteomeXchange.

Title	Neuronal GPCR NMUR-1 regulates energy homeostasis in response to pathogen infection in Caenorhabditis elegans
Description	The neuronal G protein-coupled receptor NMUR-1, a homolog to the mammalian neuromedin U receptor, has been implicated in the specificity of Caenorhabditis elegans innate immune response against pathogen infections. NMUR-1 controls C. elegans transcription activity by regulating transcription factors, which, in turn control the expression of distinct defense genes. This study further investigates the role of NMUR-1 at the protein level in regulating innate immune responses against pathogens Salmonella enterica and Enterococcus faecalis by utilizing mass spectrometry-based quantitative proteomics. We found that NMUR-1 regulates a class of proteins responsible for transmembrane transport during infection. Specifically, a group of proteins forming F1FO ATP synthase responsible for ATP biosynthesis is downregulated in NMUR-1 loss of function mutants during both S. enterica and E. faecalis infections. ATP measurements further revealed that nmur-1 mutants have reduced ATP production in response to both S. enterica and E. faecalis infections. Functional assays demonstrated that inhibiting F1FO ATP synthase using RNA interference or chemical modification mimicked the survival phenotypes of the untreated nmur-1 knockout mutants on S. enterica and E. faecalis. These findings provide valuable insights into the mechanism by which NMUR-1 regulates energy homeostasis at the protein level as part of an innate immune response against specific pathogens.
HostingRepository	PRIDE
AnnounceDate	2025-10-03
AnnouncementXML	Submission_2025-10-02_16:21:07.415.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045826
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Phillip Wibisono
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 12:50:33	ID requested
⏵ 1	2025-10-02 16:21:07	announced

10.6019/PXD045826;

submitter keyword: Caenorhabditis elegans, F1FO ATP synthase, Immunity, Pathogen Infection, G-protein coupled receptors, NMUR-1

Jingru Sun
contact affiliation	Department of Translational Medicine and Physiology, Washington State University, Spokane, WA, USA
contact email	jingru.sun@wsu.edu
lab head
Phillip Wibisono
contact affiliation	Washington State University
contact email	phillip.wibisono@wsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045826
     1. Label: PRIDE project
     2. Name: Neuronal GPCR NMUR-1 regulates energy homeostasis in response to pathogen infection in Caenorhabditis elegans