PXD045821

PXD045821 is an original dataset announced via ProteomeXchange.

Title	Intracellular Iron Accumulation Facilitates Mycobacterial Infection in Old Mouse Macrophages
Description	Aging has a significant impact on the immune system, leading to a gradual decline in immune function and changes in the body's ability to respond to bacterial infections. Non-tuberculous mycobacteria (NTM), also known as atypical mycobacteria or environmental mycobacteria, are commonly found in soil, water, and various environmental sources. While many NTM species are considered opportunistic pathogens, some can cause significant infections, particularly in individuals with compromised immune systems, such as the elderly. When mycobacteria enter the body, macrophages are among the first immune cells to encounter them, and attempt to engulf mycobacteria through a process called phagocytosis. Some NTM species, including Mycobacterium avium (M.avium) can survive and replicate within macrophages. However, little is known about the interaction between NTM and macrophages in the elderly. In this study, we investigated the mouse bone marrow-derived macrophage (BMMs) response to M. avium serotype 4, one of the main NTM species in patients with pulmonary NTM diseases. Our results demonstrated that old mouse BMMs have an increased level of intracellular iron and are more susceptible to M. avium serotype 4 infection compared to young mouse BMMs. The whole-cell proteomic analysis indicated a dysregulated expression of iron homeostasis-associated proteins in old mouse BMMs regardless of mycobacterial infection. Deferoxamine, an iron chelator, significantly rescued mycobacterial killing and phagolysosome maturation in old mouse BMMs. Therefore, our data indicate that an intracellular iron overload improves NTM survival within macrophages, and suggest a potential application of iron chelating drugs as a host-directed therapy for pulmonary NTM infection in the elderly
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:25.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Hartson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Mycobacterium avium; NCBI TaxID: 1764; scientific name: Mycobacterium tuberculosis; NCBI TaxID: NCBITaxon:1773;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 09:51:19	ID requested
1	2024-05-24 01:13:48	announced
⏵ 2	2024-10-22 06:42:31	announced	2024-10-22: Updated project metadata.

Kotey SK, Tan X, Fleming O, Kasiraju RR, Dagnell AL, Van Pelt KN, Rogers J, Hartson SD, Thadathil N, Selvarani R, Ranjit R, Logan S, Deepa SS, Richardson A, Cheng Y, Intracellular iron accumulation facilitates mycobacterial infection in old mouse macrophages. Geroscience, 46(2):2739-2754(2024) [pubmed]

10.1007/s11357-023-01048-1;

submitter keyword: macrophages,iron, mycobacterium, aging

Yong Cheng
contact affiliation	Oklahoma State University Dept. Biochemisty and Molecular Biology
contact email	ycheng@okstate.edu
lab head
Steven Hartson
contact affiliation	Oklahoma State University
contact email	hartson.steve@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045821

PRIDE project URI

• PRIDE
  1. PXD045821
     1. Label: PRIDE project
     2. Name: Intracellular Iron Accumulation Facilitates Mycobacterial Infection in Old Mouse Macrophages