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PXD045821

PXD045821 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntracellular Iron Accumulation Facilitates Mycobacterial Infection in Old Mouse Macrophages
DescriptionAging has a significant impact on the immune system, leading to a gradual decline in immune function and changes in the body's ability to respond to bacterial infections. Non-tuberculous mycobacteria (NTM), also known as atypical mycobacteria or environmental mycobacteria, are commonly found in soil, water, and various environmental sources. While many NTM species are considered opportunistic pathogens, some can cause significant infections, particularly in individuals with compromised immune systems, such as the elderly. When mycobacteria enter the body, macrophages are among the first immune cells to encounter them, and attempt to engulf mycobacteria through a process called phagocytosis. Some NTM species, including Mycobacterium avium (M.avium) can survive and replicate within macrophages. However, little is known about the interaction between NTM and macrophages in the elderly. In this study, we investigated the mouse bone marrow-derived macrophage (BMMs) response to M. avium serotype 4, one of the main NTM species in patients with pulmonary NTM diseases. Our results demonstrated that old mouse BMMs have an increased level of intracellular iron and are more susceptible to M. avium serotype 4 infection compared to young mouse BMMs. The whole-cell proteomic analysis indicated a dysregulated expression of iron homeostasis-associated proteins in old mouse BMMs regardless of mycobacterial infection. Deferoxamine, an iron chelator, significantly rescued mycobacterial killing and phagolysosome maturation in old mouse BMMs. Therefore, our data indicate that an intracellular iron overload improves NTM survival within macrophages, and suggest a potential application of iron chelating drugs as a host-directed therapy for pulmonary NTM infection in the elderly
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:42:25.755.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Hartson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Mycobacterium avium; NCBI TaxID: 1764; scientific name: Mycobacterium tuberculosis; NCBI TaxID: NCBITaxon:1773;
ModificationListdeamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-29 09:51:19ID requested
12024-05-24 01:13:48announced
22024-10-22 06:42:31announced2024-10-22: Updated project metadata.
Publication List
Kotey SK, Tan X, Fleming O, Kasiraju RR, Dagnell AL, Van Pelt KN, Rogers J, Hartson SD, Thadathil N, Selvarani R, Ranjit R, Logan S, Deepa SS, Richardson A, Cheng Y, Intracellular iron accumulation facilitates mycobacterial infection in old mouse macrophages. Geroscience, 46(2):2739-2754(2024) [pubmed]
10.1007/s11357-023-01048-1;
Keyword List
submitter keyword: macrophages,iron, mycobacterium, aging
Contact List
Yong Cheng
contact affiliationOklahoma State University Dept. Biochemisty and Molecular Biology
contact emailycheng@okstate.edu
lab head
Steven Hartson
contact affiliationOklahoma State University
contact emailhartson.steve@gmail.com
dataset submitter
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Dataset FTP location
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