PXD045811

PXD045811 is an original dataset announced via ProteomeXchange.

Title	UV-A receptor CRY-DASH1 up- and UV-A receptor CRY-DASH1 up- and downregulates proteins involved in different plastidial pathways
Description	Algae encode up to five different types of cryptochrome photoreceptors. So far, relatively little is known about the biological functions of the so-called DASH (Drosophila, Arabidopsis, Synechocystis and Homo)-type cryptochromes. The green alga Chlamydomonas reinhardtii encodes two of them. CRY-DASH1 also called DCRY1 has its maximal absorption peak in the UV-A range. It is localized in the chloroplast and plays an important role in balancing the photosynthetic machinery. Here, we performed a comparative analysis of chloroplast proteins from wild type and a knockout mutant of CRY-DASH1 named cry-dash1mut, using label-free quantitative proteomics as well as immunoblotting. Our results show upregulation of enzymes involved in specific pathways in the mutant including key enzymes of chlorophyll and carotenoid biosynthesis consistent with increased levels of photosynthetic pigments in cry-dash1mut. There is also an increase in certain redox as well as photosystem I and II proteins, including D1. Strikingly, CRY-DASH1 is coregulated in a D1 deletion mutant, where its amount is increased. In contrast, key proteins of the central carbon metabolism, including glycolysis/gluconeogenesis, dark fermentation and the oxidative pentose pathway are downregulated in cry-dash1mut. Similarly, enzymes of histidine biosynthesis are downregulated in cry-dash1mut leading to a reduction in the amount of free histidine. Yet, transcripts encoding for several of these proteins are at a similar level in the wild type and cry-dash1mut or even opposite. We show that CRY-DASH1 can bind to RNA, taking the the psbA RNA encoding D1 as target. These data suggest that CRY-DASH1 regulates plastidial metabolic pathways at the post-transcriptional level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:40.521.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045811
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Volker Wagner
SpeciesList	scientific name: Chlamydomonas reinhardtii CC3269; NCBI TaxID: 906914;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 06:52:46	ID requested
1	2023-10-02 09:01:28	announced
⏵ 2	2023-11-14 09:05:47	announced	2023-11-14: Updated project metadata.

Rredhi A, Petersen J, Wagner V, Vuong T, Li W, Li W, Schrader L, Mittag M, The UV-A Receptor CRY-DASH1 Up- and Downregulates Proteins Involved in Different Plastidial Pathways. J Mol Biol, 436(5):168271(2024) [pubmed]

10.6019/PXD045811;

submitter keyword: photoreceptor, central carbon metabolism, chlorophyll biosynthesis, histidine

Maria Mittag
contact affiliation	General Botany, Matthias-Schleiden-Institut, Friedrich-Schiller University Jena Am Planetarium 1, 07743 Jena, Germany
contact email	M.Mittag@uni-jena.de
lab head
Volker Wagner
contact affiliation	Matthias Schleiden-Institute, General Botany, Friedrich Schiller-University Jena Am Planetarium 1, 07743 Jena
contact email	Volker.Wagner@uni-jena.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045811

PRIDE project URI

• PRIDE
  1. PXD045811
     1. Label: PRIDE project
     2. Name: UV-A receptor CRY-DASH1 up- and UV-A receptor CRY-DASH1 up- and downregulates proteins involved in different plastidial pathways