PXD045801

PXD045801 is an original dataset announced via ProteomeXchange.

Title	A YTHDF-PABP axis is required for m6A-mediated organogenesis in plants
Description	N6-methyladenosine (m6A) in mRNA is key to eukaryotic gene regulation. Many m6A functions involve RNA-binding proteins that recognize m6A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction with protein partners, but whose precise biochemical functions remain largely unknown. The Arabidopsis thaliana YTH domain proteins ECT2, ECT3 and ECT4 accelerate organogenesis through stimulation of cell division in organ primordia. Here, we use ECT2 to reveal molecular underpinnings of this function. We show that stimulation of leaf formation requires the long N-terminal IDR, and we identify two short IDR-elements required for ECT2-mediated organogenesis. Of these two, a 19-amino acid region containing a tyrosine-rich motif conserved in both plant and metazoan YTHDF proteins is necessary for binding to the major cytoplasmic poly(A)-binding proteins PAB2, PAB4 and PAB8. Remarkably, overexpression of PAB4 in leaf primordia partially rescues the delayed leaf formation in ect2 ect3 ect4 mutants, suggesting that the ECT2-PAB2/4/8 interaction on target mRNAs of organogenesis-related genes may overcome limiting PAB concentrations in primordial cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:16:28.625.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathias Tankmar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 00:49:52	ID requested
1	2023-11-28 05:29:05	announced
⏵ 2	2024-10-22 06:16:29	announced	2024-10-22: Updated project metadata.

10.15252/embr.202357741;

Due Tankmar M, Reichel M, Arribas-Hern, á, ndez L, Brodersen P, A-mediated organogenesis in plants. EMBO Rep, 24(12):e57741(2023) [pubmed]

submitter keyword: Arabidopsis, Plants,m6A, ECT2

Peter Brodersen
contact affiliation	Department of Computational and RNA Biology, Copenhagen University, Denmark
contact email	pbrodersen@bio.ku.dk
lab head
Mathias Tankmar
contact affiliation	Copenhagen University
contact email	mathias.tankmar@bio.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045801
     1. Label: PRIDE project
     2. Name: A YTHDF-PABP axis is required for m6A-mediated organogenesis in plants