PXD045797

PXD045797 is an original dataset announced via ProteomeXchange.

Title	Enhanced Secretion of Promyogenic Small Extracellular Vesicles/Exosomes by Quiescent Muscle Cells
Description	Skeletal muscle tissue plays a key metabolic role in the organism, partly via signaling to other tissues. Intercellular signaling via small extracellular vesicles (sEV) is emerging as an important mechanism in homeostasis, regeneration and disease, but the roles of sEV derived from muscle cells are poorly defined. In a culture model of physiologically relevant muscle cell states, we observed quantitative differences in secretion of sEV with size ~150 nm and marker profile (Alix, TSG101, flotillin-1, CD9) consistent with exosomes. Unexpectedly, rates of sEV secretion were reversibly increased as myoblasts entered and exited G0, correlating with expression of Kibra, a regulator of EV biogenesis. Perturbation of Kibra led to corresponding alterations of sEV secretion, supporting a role in enhanced sEV biogenesis in G0. Proteomic profiling of sEV revealed a set of proteins common to all muscle cell states, as well as state-specific proteins. Functionally, donor sEV purified from all muscle cell states were able to activate a Wnt reporter in target cells, suggesting preservation of signaling capability. However, only G0-derived sEV were able to induce endogenous myogenic markers and phenotypic differentiation to myotubes. Taken together, we provide evidence that quiescence in myogenic cells is associated with quantitative and qualitative alterations in sEV secretion and signaling function. Given the enhanced secretion and unique signaling properties of sEV from G0 muscle cells in culture, we propose that sEV produced by quiescent muscle stem cells may play an important role in vivo, during muscle homeostasis and regeneration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:49:14.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Swasti Raychaudhuri
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 22:59:15	ID requested
1	2024-07-08 23:24:17	announced
⏵ 2	2024-10-22 06:49:14	announced	2024-10-22: Updated project metadata.

10.3389/FCELL.2024.1381357;

submitter keyword: exosomes, Wnt, myoblast, sEV, differentiation, G0, KIBRA, promyogenic,Quiescence

Jyotsna Dhawan
contact affiliation	Emeritus Scientist, CSIR-Centre for Cellular and Molecular Biology (CCMB)
contact email	jdhawan@ccmb.res.in
lab head
Swasti Raychaudhuri
contact affiliation	CSIR-Centre for Cellular and Molecular Biology
contact email	rcswasti@ccmb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD045797

PRIDE project URI

• PRIDE
  1. PXD045797
     1. Label: PRIDE project
     2. Name: Enhanced Secretion of Promyogenic Small Extracellular Vesicles/Exosomes by Quiescent Muscle Cells