PXD045796

PXD045796 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence
Description	Here, we describe a secreted HLA (sHLA) Fc-fusion construct for simple single HLA allele profiling in hypoxic pancreatic ductal adenocarcinoma (PDAC) and cellular senescence. This method streamlines sample preparation, enables temporal control, and provides allele-restricted target identification. Over 30,000 unique HLA-associated peptides were identified across two different HLA alleles and seven cell lines, with ~9,300 peptides newly discovered. The sHLA Fc-fusion capture technology holds potential to expedite immunopeptidomics and advance therapeutic interest in peptide-HLA complexes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_13:54:40.579.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa Kirkemo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 21:07:47	ID requested
⏵ 1	2024-01-26 13:54:41	announced

Publication List

10.1093/pnasnexus/pgad400;
Rettko NJ, Kirkemo LL, Wells JA, Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence. PNAS Nexus, 2(12):pgad400(2023) [pubmed]

10.1093/pnasnexus/pgad400;

Rettko NJ, Kirkemo LL, Wells JA, Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence. PNAS Nexus, 2(12):pgad400(2023) [pubmed]

Keyword List

submitter keyword: Immunopeptidomics, Senescence, Secreted HLA, Hypoxia

submitter keyword: Immunopeptidomics, Senescence, Secreted HLA, Hypoxia

Contact List

Jim Wells
contact affiliation	Department of Pharmaceutical Chemistry School of Pharmacy UC San Francisco
contact email	jim.wells@ucsf.edu
lab head
Lisa Kirkemo
contact affiliation	UC San Francisco
contact email	lkirkemo@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045796
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045796

PRIDE project URI

Repository Record List

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