PXD045794

PXD045794 is an original dataset announced via ProteomeXchange.

Title	Regulation of mitochondrial function and protein homeostasis by hypoxia inducible factor 1 alpha in normoxia-mouse cells
Description	Hypoxia inducible factors (HIF), specifically, HIF1A, is continuously synthesized and degraded rapidly in normoxia. During hypoxia, HIF1A stabilization limits oxygen utilization and inhibits protein synthesis, but there are limited data on HIF1A function(s) in normoxia. Given the high energy needs of contraction and maintenance of the large protein mass in skeletal muscle, we determined HIF1A function in normoxia in differentiated murine myotubes with loss/gain of function and skeletal muscle from mice with Hif1afl/f or post-natal muscle-specific deletion (Hif1amsd). Integration of transcriptomics and proteomics in myotubes and muscle from mice with Hif1amsd showed enrichment of mitochondrial/metabolic regulatory molecules including TCA cycle and electron transport chain components. Mitochondrial oxidative functions and ATP content were higher with less free radical generation with Hif1a deletion. Untargeted metabolomics showed enrichment in TCA cycle components and senescence regulation. Targeted metabolomics showed higher concentrations of most TCA cycle intermediates and expression of sirtuin 3, with less abundance of markers of post-mitotic senescence. Under normoxia, regulation of mitochondrial oxidation and post-mitotic senescence in skeletal muscle by the transient expression of HIF1A may have relevance in other tissues also.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_19:13:05.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 15:33:27	ID requested
⏵ 1	2025-06-08 19:13:06	announced

Welch N, Mishra S, Bellar A, Kannan P, Gopan A, Goudarzi M, King J, Luknis M, Musich R, Agrawal V, Bena J, Koch CJ, Li L, Willard B, Shah YM, Dasarathy S, in normoxia. J Physiol, 602(12):2763-2806(2024) [pubmed]

10.1113/jp285339;

submitter keyword: normoxia, HIF1A,skeletal muscle, senescence

Srinivasan Dasarathy
contact affiliation	Department of Inflammation and Immunity, Gastroenterology and Hepatology. Cleveland Clinic, Cleveland Ohio, USA
contact email	dasaras@ccf.org
lab head
Ling Li
contact affiliation	Cleveland Clinic
contact email	lil5@ccf.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD045794

PRIDE project URI

• PRIDE
  1. PXD045794
     1. Label: PRIDE project
     2. Name: Regulation of mitochondrial function and protein homeostasis by hypoxia inducible factor 1 alpha in normoxia-mouse cells