Updated project metadata. ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2. Enriched proteins were analyzed by LC-MS/MS. Non-transgenic Col-0 plants served as control.