PXD045771

PXD045771 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Time-course of ischemia-reperfusion in a pig model
Description	We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the pig ischemic heart tissue during the first 24h after I/R. We used 40 castrated male Large-White pigs weighing 30 to 40 kg. Closed-chest 40 min I/R was performed in 36 animals and they were sacrificed at 20 min, 40 min, 80 minutes, 2 hours, 6 hours, 12 hours and 24 hours after reperfusion after reperfusion (n = 4 per group). 4 animals were sacrificed with no intervention other than baseline cardiac magnetic resonance ( CMR), and served as controls. A new group of 4 animals were sacrificed at 120 minutes after artery occlusion without reperfusion, and other group of 4 animals were subjected to ischemic preconditioning prior to I/R and sacrificed at 24h. Myocardial tissue samples from ischemic area were collected for proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:37.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inmaculada Jorge
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	methylthiolated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 06:43:24	ID requested
1	2024-05-24 02:47:15	announced
⏵ 2	2024-10-22 06:42:38	announced	2024-10-22: Updated project metadata.

Publication List

10.3390/antiox13010106;
Binek A, Castans C, Jorge I, Bagwan N, Rodr, í, guez JM, Fern, á, ndez-Jim, é, nez R, Gal, á, n-Arriola C, Oliver E, G, ó, mez M, Clemente-Morag, ó, n A, Ibanez B, Camafeita E, V, á, zquez J, Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury. Antioxidants (Basel), 13(1):(2024) [pubmed]

10.3390/antiox13010106;

Binek A, Castans C, Jorge I, Bagwan N, Rodr, í, guez JM, Fern, á, ndez-Jim, é, nez R, Gal, á, n-Arriola C, Oliver E, G, ó, mez M, Clemente-Morag, ó, n A, Ibanez B, Camafeita E, V, á, zquez J, Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury. Antioxidants (Basel), 13(1):(2024) [pubmed]

Keyword List

submitter keyword: PTMs, ischemic heart tissue, ischemia reperfusion (I/R),quantitative proteomics, pig

submitter keyword: PTMs, ischemic heart tissue, ischemia reperfusion (I/R),quantitative proteomics, pig

Contact List

Jesús Vázquez Cobos
contact affiliation	Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain
contact email	jesus.vazquez@cnic.es
lab head
Inmaculada Jorge
contact affiliation	Vascular Biology and Inflammation
contact email	ijorge@cnic.es
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045771
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045771

PRIDE project URI

Repository Record List

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