PXD045765

PXD045765 is an original dataset announced via ProteomeXchange.

Title	Peptidoglycan from S. aureus digested with mutanolysin and lysostaphin catalytic domain
Description	(Manuscript abstract) "Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently."
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:35.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alicja Razew
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 04:10:02	ID requested
1	2023-11-06 00:28:02	announced
2	2023-11-14 08:13:34	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:36	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-42506-W;

ProteomeXchange project tag: Glycoproteomics (B/D-HPP)

submitter keyword: peptidoglycan, S. aureus, mutanolysin

Izabela Sabala
contact affiliation	Mossakowski Medical Research Institute Polish Academy of Sciences
contact email	isabala@imdik.pan.pl
lab head
Alicja Razew
contact affiliation	Institut de Biologie Structurale
contact email	alicja.razew@ibs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045765
     1. Label: PRIDE project
     2. Name: Peptidoglycan from S. aureus digested with mutanolysin and lysostaphin catalytic domain