PXD045749

PXD045749 is an original dataset announced via ProteomeXchange.

Title	Proteome-Wide Deconvolution of Drug Targets and Binding Sites by Lysine Reactivity Profiling
Description	Recently, numerous efforts have been devoted to identifying drug targets and binding sites in complex proteomes, which is of great importance in modern drug discovery. In this study, we developed a robust lysine reactivity profiling method to systematically study drug-binding targets and binding sites at the proteome level. This method is based on the principle that binding of a drug to a specific region of target proteins will change the reactivity of lysine residues that are located at this region, and these changes can be detected with an enrichable and lysine reactive probe. Coupled with data-independent acquisition (DIA), the known target proteins and corresponding binding sites were successfully revealed from K562 cell lysates for three model drugs: geldanamycin, staurosporine, and dasatinib. In addition, the drug-induced conformational changes of certain targets were also revealed by our method during the screening of staurosporine. The screening sensitivity of our method revealed from the screening of stuarosporine and dasatinib was comparable with that of thermal proteome profiling (TPP) or machine learning-based limited proteolysis (LiP-Quant). Overall, 21 and 4 kinase targets, including adenosine 5′-triphosphate (ATP)-binding targets, were identified for staurosporine and dasatinib in K562 cell lysates, respectively. We found that target proteins identified by TPP, LiP-Quant, and our method were complementary, emphasizing that the development of new methods that probe different properties of proteins is of great importance in drug target deconvolution. We also envision further applications of our method in proteome-wide probing multiple events that involve lysine reactivity changes.
HostingRepository	iProX
AnnounceDate	2023-09-28
AnnouncementXML	Submission_2023-09-27_21:45:23.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheng Fang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 21:45:15	ID requested
⏵ 1	2023-09-27 21:45:24	announced

Ruan C, Zhou J, Li Z, Li K, Fang Z, Zhang X, Ye M, Proteome-Wide Deconvolution of Drug Targets and Binding Sites by Lysine Reactivity Profiling. Anal Chem, 94(7):3352-3359(2022) [pubmed]

submitter keyword: Drug Target

Mingliang Ye
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	mingliang@dicp.ac.cn
lab head
Zheng Fang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	zhengfang@dicp.ac.cn
dataset submitter

iProX dataset URI