PXD045740
PXD045740 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bifurcated malarial translation elongation cycle |
| Description | Malaria parasites rely heavily on rapid, high fidelity protein synthesis to infect and replicate in human erythrocytes, making translation an attractive target for new anti-malarials. Unfortunately, our knowledge of the inner workings of translation inside the cell remain limited. Here, we have determined native structures of Pf80S ribosomes in multiple conformational and compositional states in situ, from cryoFIB-milled Plasmodium falciparum-infected human erythrocytes. We mapped the distributions of ribosomes among these states, defining the translational landscape across the morphologically distinct stages of the parasite asexual intraerythrocytic replication cycle. We then employed an interdisciplinary, multi-scaled approach to interrogate how inhibiting translation perturbs the distribution of ribosomes among these states and to elucidate the corresponding molecular, ultrastructural and cellular consequences. We observe a relatively uniform distribution of Pf80S ribosomes across the intermediate states of translation elongation in the cell, in contrast with what is observed in other organisms. Furthermore, we find two translation intermediates co-exist in trophozoite and schizont stage parasites that have not been seen together in other organisms thus far. Our work provides a deeper understanding of malarial protein synthesis, informing development of new therapies. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-05-07 |
| AnnouncementXML | Submission_2025-05-07_17:58:55.511.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | TMTpro; Phospho |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-27 10:47:32 | ID requested | |
| ⏵ 1 | 2025-05-07 17:58:55 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Pf80S ribosome, Malaria, in situ cryoET |
Contact List
| Chi-Min Ho | |
|---|---|
| contact affiliation | Columbia University Irving Medical Center |
| contact email | chi-min.ho@columbia.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000092987/ |
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