PXD045730

PXD045730 is an original dataset announced via ProteomeXchange.

Title	Proteome dysregulation induced by degrader peptides targeting GroEL chaperone in Escherichia coli
Description	Inducible degradation presents an attractive alternative for modulating protein levels in bacteria without genome editing or RNA interference. In this work we constructed a series of plasmids encoding degrader peptides which bring together an essential GroEL chaperone and activated ClpXP protease to trigger GroEL degradation. Escherichia coli BW25113 was transformed with studied and control plasmids. The effect of peptide expression on bacterial proteome was monitored in thermal stress (42 °C) by TMT-MS in three time points to confirm GroEL degradation and secondary effects on bacterial proteome arising from target depletion.
HostingRepository	PRIDE
AnnounceDate	2025-06-24
AnnouncementXML	Submission_2025-06-24_03:51:34.895.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 06:52:38	ID requested
⏵ 1	2025-06-24 03:51:35	announced

10.1038/S44319-025-00510-9;

submitter keyword: targeted protein degradation,GroEL, peptide, degrader, proteostasis

Maria Górna
contact affiliation	Structural Biology Group, Biological and Chemical Research Centre, Department of Chemistry, University of Warsaw, Warsaw, Poland
contact email	mgorna@chem.uw.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045730
     1. Label: PRIDE project
     2. Name: Proteome dysregulation induced by degrader peptides targeting GroEL chaperone in Escherichia coli