PXD045726

PXD045726 is an original dataset announced via ProteomeXchange.

Title	Comparison of proteins identified using the Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) versus the non-functional (G42E) Macro domain for enrichment of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2 (DDA method)
Description	ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2. A non-functional Af1521G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:24.168.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Wirthmueller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue; phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 04:14:14	ID requested
1	2023-09-27 23:58:07	announced
⏵ 2	2023-11-14 08:46:24	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, type III effector, Pseudomonas syringae, plant pathogen,ADP-ribosylation

Lennart Wirthmueller
contact affiliation	Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
contact email	lennart.wirthmueller@ipb-halle.de
lab head
Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry Dept. Biochemistry of Plant Interactions Weinberg 3 06120 Halle (Saale)
contact email	lennart.wirthmueller@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045726
     1. Label: PRIDE project
     2. Name: Comparison of proteins identified using the Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) versus the non-functional (G42E) Macro domain for enrichment of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2 (DDA method)