PXD045721

PXD045721 is an original dataset announced via ProteomeXchange.

Title	Single blood donor causing multiple allergic and anaphylactic transfusion reactions
Description	Allergic transfusion reactions (ATRs) occur within 24 h of transfusion. A less common but more severe manifestation of ATR is anaphylaxis. The relationship between the mechanisms underlying ATRs and donor, product, and/or recipient factors is not well understood. Our aim is to investigate the underlying factors responsible for eliciting anaphylactic ATRs and possibly identify the molecule responsible, that could routinely be used as a biomarker in subsequent pre-transfusion screens. We employ an IP-MS workflow for this purpose. Purified IgG antibodies from donor plasma are immobilized on magnetic beads and interacted with basophil cells lysates. The immunoprecipitants are analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:30:26.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-26 23:09:53	ID requested
⏵ 1	2026-06-07 16:30:27	announced

10.3324/haematol.2025.288783;

Brooks PT, Mikkelsen C, Bartko EA, Ravikumar V, Hother CE, Minculescu L, Vibeke H, Ostrowski SR, Hansen MB, Poulsen LK, Blom LH, Garvey LH, Dziegiel MH, Severe allergic and anaphylactic transfusion reactions in consecutive recipients from the same donor. Haematologica, 111(5):1822-1827(2026) [pubmed]

submitter keyword: acute transfusion reaction,Anaphylaxis, mass spectrometry, immunoprecipitation

Morten Hanefeld Dziegiel
contact affiliation	Blodbanken KI2034, Klinisk Immunologisk afdeling, Rigshospitalet (Copenhagen University Hospital, Denmark), Blegdamsvej 9, 2100 København Ø (Copenhagen)
contact email	Morten.Dziegiel@regionh.dk
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD045721

PRIDE project URI

• PRIDE
  1. PXD045721
     1. Label: PRIDE project
     2. Name: Single blood donor causing multiple allergic and anaphylactic transfusion reactions