PXD045705

PXD045705 is an original dataset announced via ProteomeXchange.

Title	Insights into enhancing Centella asiatica organ cell biofactories via hairy root protein profiling
Description	The objectives of this study encompassed a thorough exploration of the potential implications of protein profiling in hairy roots, specifically focusing on optimizing and enhancing C. asiatica organ cell biofactories. In this pursuit, we categorized established C. asiatica hairy root lines according to their capacity for centelloside production, classifying them into HIGH, MID, or LOW categories. For comparative analysis, wild adventitious (Adv) roots were extracted from in vitro C. asiatica seedlings and cultivated in solid MS medium at 25°C in complete darkness, serving as control specimens. This meticulous, label-free proteomic analysis enabled the successful identification of several proteins. Our research substantially builds upon and extends the findings presented by Alcalde et al. (2022) (DOI: 10.3389/fpls.2022.1001023). In their study, distinctive morphological and metabolic variations were noted among different C. asiatica hairy root lines. Such differences are presumably attributable to the random incorporation of a selective set of genes from the T-DNA, with particular emphasis on the rol and aux genes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_08:15:05.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diego Alberto Hidalgo Martinez
SpeciesList	scientific name: Centella asiatica; NCBI TaxID: 48106;
ModificationList	iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-26 10:50:53	ID requested
⏵ 1	2024-01-26 08:15:06	announced

Alcalde MA, Hidalgo-Martinez D, Bru Mart, í, nez R, Sell, é, s-Marchart S, Bonfill M, Palazon J, organ cell biofactories via hairy root protein profiling. Front Plant Sci, 14():1274767(2023) [pubmed]

10.3389/fpls.2023.1274767;

submitter keyword: Centella asiatica, plant biotechnology, molecular farming, Centelloside production,Rhizobium rhizogenes, biomarkers

Diego A. Hidalgo
contact affiliation	Dpt. Biologia, Sanidad y Medio ambiente Fac. Farmacia y Ciencias de la Alimentacion Univ. Barcelona Av.Joan XIII, 27-31 08028 Barcelona SPAIN
contact email	dhidalgo@ub.edu
lab head
Diego Alberto Hidalgo Martinez
contact affiliation	University of Barcelona
contact email	dhidalgo@ub.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045705

PRIDE project URI

• PRIDE
  1. PXD045705
     1. Label: PRIDE project
     2. Name: Insights into enhancing Centella asiatica organ cell biofactories via hairy root protein profiling