PXD045691
PXD045691 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A MALDI-TOF MS based method for the fast screening of recombinant protein high-yield strains |
| Description | We presented a MALDI-TOF MS based approach for the screening of recombinant protein high-yield strains. It relies on the MALDI MS analysis of the affinity tag containing peptide that released from the target recombinant proteins expressed in host strains. This approach was illustrated by quantifying the His-tag containing target peptide which was removed from the recombinant protein of Src Homology 2 superbinder by tobacco etch virus protease. After the specific enrichment and detection of target peptide with the theoretical molecular weight of 1895.09 Da, we could achieve the identification of strains with different expression levels of Src Homology 2 superbinder. Furthermore, the screening of strains at the absolute quantitative level was realized by adding the synthesized heavy labeled peptide as internal standards, and the quantitative results have a good linear correlation. In general, based on the virtue of high speed, accuracy and sensitivity of MALDI-TOF MS, this approach has the potential to achieve high throughput detection, and then provide powerful technical support for the screening of recombinant protein high-yield strains. |
| HostingRepository | iProX |
| AnnounceDate | 2023-09-26 |
| AnnouncementXML | Submission_2023-09-26_04:13:59.873.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zheng Fang |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | 5800 TOF/TOF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-26 04:13:38 | ID requested | |
| ⏵ 1 | 2023-09-26 04:14:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Recombinant Protein |
Contact List
| Mingliang Ye | |
|---|---|
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | mingliang@dicp.ac.cn |
| lab head | |
| Zheng Fang | |
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | zhengfang@dicp.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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