PXD045683
PXD045683 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Traceless peptide and protein modification via rational tuning of halopyridiniums |
| Description | Building on the insights gained from systematically investigating of aromatic nucleophilic substitution (SNAr) and thiol-exchange, we identified the highly electron-deficient halopyridiniums as a tunable and predictable reaction platform for adjusting electrophilicity and thiol-cleavability by following a positive Hammett correlation. This pyridinium-based platform was practical for both cleavable peptide stapling and protein functionalization. With this highly tunable and predictable toolkit, we successfully achieved GSH-response cleavable peptide stapling, antibody conjugation, enzyme masking/de-masking, and proteome labeling. Furthermore, the positively charged species exhibit improved cell-permeability and digestion-stability, making them attractive candidates for the manipulation of the functions and biophysical properties of biomolecules. |
| HostingRepository | iProX |
| AnnounceDate | 2023-09-25 |
| AnnouncementXML | Submission_2024-05-21_00:51:58.742.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rui Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-25 20:23:29 | ID requested | |
| 1 | 2023-09-25 20:23:46 | announced | |
| ⏵ 2 | 2024-05-21 00:51:59 | announced | 2024-05-21: Update information. |
Publication List
| Wan C, Zhang Y, Wang J, Xing Y, Yang D, Luo Q, Liu J, Ye Y, Liu Z, Yin F, Wang R, Li Z, Traceless Peptide and Protein Modification via Rational Tuning of Pyridiniums. J Am Chem Soc, 146(4):2624-2633(2024) [pubmed] |
Keyword List
| submitter keyword: Halopyridinium modification, Protein profiling, Cleavable peptide stapling |
Contact List
| Rui Wang | |
|---|---|
| contact affiliation | Pingshan Translational Medicine Center, Shenzhen Bay Laboratory |
| contact email | wangrui@szbl.ac.cn |
| lab head | |
| Rui Wang | |
| contact affiliation | Pingshan Translational Medicine Center, Shenzhen Bay Laboratory |
| contact email | wangrui@szbl.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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