PXD045674

PXD045674 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The CRTC-1 transcriptional domain is required for COMPASS complex mediated longevity in C. elegans
Description	Loss of function during ageing is accompanied by transcriptional drift, altering gene expression and contributing to a variety of age-related diseases. CREB-regulated transcriptional coactivators (CRTCs) have emerged as key regulators of gene expression that might be targeted to promote longevity. Here, we define the role of the Caenorhabditis elegans CRTC-1 in the epigenetic regulation of longevity. Endogenous CRTC-1 binds chromatin factors, including components of the COMPASS complex, which trimethylates lysine 4 on histone H3 (H3K4me3). CRISPR editing of endogenous CRTC-1 reveals that the CREB-binding domain in neurons is specifically required for H3K4me3-dependent longevity. However, this effect is independent of CREB but instead acts via the transcription factor AP-1. Strikingly, CRTC-1 also mediates global histone acetylation levels, and this acetylation is essential for H3K4me3-dependent longevity. Indeed, overexpression of an acetyltransferase enzyme is sufficient to promote longevity in wild-type worms. CRTCs, therefore, link energetics to longevity by critically fine-tuning histone acetylation and methylation to promote healthy ageing.
HostingRepository	jPOST
AnnounceDate	2023-09-26
AnnouncementXML	Submission_2023-09-25_14:42:51.747.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CARLOS G SILVA GARCIA
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N6-acetyl-L-lysine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-25 14:42:48	ID requested
⏵ 1	2023-09-25 14:42:52	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: CRTC-1, Aging, COMPASS, Epigenetics, IP

submitter keyword: CRTC-1, Aging, COMPASS, Epigenetics, IP

Contact List

William B. Mair
lab head
CARLOS G SILVA GARCIA
contact affiliation	Brown University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002331/