PXD045673

PXD045673 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Human Adenovirus B and C infections in the presence of interferon
Description	Human adenoviruses (HAdV) are a large family of DNA viruses generally infecting the airway, the gut, the eye, and the urinary tract. However, certain strains of HAdVs are capable of causing severe infections in the very young, the elderly, the immunocompromised, and sometimes healthy individuals. In particular, species B HAdVs are often associated with more severe disease, involving serotype 7 and 14. Despite the severity of the disease caused by these strains, little is known about the molecular mechanisms underpinning the disease outcomes. Activation of interferon stimulated genes (ISGs) occurs rapidly after interferon (IFN) stimulation and relies on recruitment of the transcription factor complex IFN-stimulated gene factor 3 (ISGF3) to promoters, which happens via the canonical JAK-STAT pathway. Therefore, suppression of IFNs is a key aspect of evading innate immunity by viral pathogens, including HAdVs; and as such, HAdVs have evolved multiple ways by which they suppress activation of ISGs in order to drive viral replication. Our previous work investigated the molecular mechanism of interferon suppression by HAdV-C5. We observed that E1A of HAdV-C5 interacts with the AAA+ DNA helicase RuvBL1/Pontin in order to suppress activation of interferon stimulated genes (ISGs) during infection. We therefore wanted to determine whether RuvBL1 is also a target for the more highly pathogenic HAdV-B7 and B14. As part of this investigation, we examined the broad-range effect of HAdV species C2, B7, and B14 on cells in response to interferon. These results are presented here.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:28.465.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045673
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Drayson Graves
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-25 14:27:27	ID requested
1	2024-06-26 06:54:51	announced
⏵ 2	2024-10-22 06:47:28	announced	2024-10-22: Updated project metadata.

10.6019/PXD045673;

10.1128/MBIO.01038-24;

submitter keyword: Human, ISGs, Interferon, Mastadenovirus, HAdV-C2, HAdV-B7, HAdV-B14, Adenovirus

Peter Pelka
contact affiliation	University of Manitoba
contact email	peter.pelka@umanitoba.ca
lab head
Drayson Graves
contact affiliation	University of Manitoba
contact email	draysongraves@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045673

PRIDE project URI

• PRIDE
  1. PXD045673
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Human Adenovirus B and C infections in the presence of interferon