PXD045647 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Effect of malignant ascites before and after therapy on the proteome of ovarian cancer cells |
Description | Ovarian cancer is characterized by transcoelomic metastasis into the peritoneal cavity. The peritoneal malignant ascites is enriched with ovarian cancer cells and a small amount of tumor-associated immune cells which create a unique microenvironment actively contributing to progression of the disease. However, it remains unclear how cancer cells communicate to its local environment under the influence of chemotherapy. To address this issue, we performed LC-MS/MS analyses of several primary cultures of ovarian cancer cells from chemonaive malignant ascites incubated for 3 days with autologous pre- or post-chemotherapy ascitic fluids. Enrichment analysis identified prominent upregulation of proteins associated with pathways of DNA repair and cell cycle regulation in tumor cells incubated with ascitic fluids after chemotherapy. Consistently with these data, ascites after therapy increased the resistance of ovarian cancer cells to cisplatin. These findings demonstrate that under stress condition cancer cells can secrete signaling molecules into the extracellular space and contribute to the emergence of tumor chemoresistance during short time period. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-29 |
AnnouncementXML | Submission_2024-05-29_08:58:46.344.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD045647 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Georgij Arapidi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-25 04:46:54 | ID requested | |
⏵ 1 | 2024-05-29 08:58:46 | announced | |
Publication List
Keyword List
submitter keyword: Ovarian Cancer, Chemoresistance, Ascites |
Contact List
Georgij Arapidi |
contact affiliation | System Biology Laboratory at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
contact email | arapidi@gmail.com |
lab head | |
Georgij Arapidi |
contact affiliation | Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
contact email | arapidi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045647 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045647
- Label: PRIDE project
- Name: Effect of malignant ascites before and after therapy on the proteome of ovarian cancer cells