PXD045630
PXD045630 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human serum glycoproteome - Glyco-Decipher |
Description | Identification results of peptide-spectrum matches supporting Glyco-Decipher manuscript (Glyco-Decipher: Glycan database-independent peptide matching enables discovery of new glycans and in-depth characterization of site-specific N-glycosylation). Recently, several elegant bioinformatics tools have been developed to identify glycopeptides from tandem mass spectra for site-specific glycoproteomics studies. These glycan database-dependent tools have substantially improved glycoproteomics analysis but fail to identify glycopeptides with unexpected glycans. We present a platform called Glyco-Decipher to interpret the glycoproteomics data of N-linked glycopeptides. It adopts a glycan database-independent peptide matching scheme that allows the unbiased profiling of glycans and the discovery of new glycans linked with modifications. Reanalysis of several large-scale datasets showed that Glyco-Decipher outperformed the open search method in glycan blind searching and the popular glycan database-dependent software tools in glycopeptide identification. Our glycan database-independent search also revealed that modified glycans are responsible for a large fraction of unassigned glycopeptide spectra in shotgun glycoproteomics. |
HostingRepository | iProX |
AnnounceDate | 2023-09-25 |
AnnouncementXML | Submission_2023-09-24_23:31:51.809.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zheng Fang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-09-24 23:30:38 | ID requested | |
⏵ 1 | 2023-09-24 23:31:52 | announced |
Publication List
Fang Z, Qin H, Mao J, Wang Z, Zhang N, Wang Y, Liu L, Nie Y, Dong M, Ye M, Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation. Nat Commun, 13(1):1900(2022) [pubmed] |
Keyword List
submitter keyword: Glyco-Proteomics |
Contact List
Mingliang Ye | |
---|---|
contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
contact email | mingliang@dicp.ac.cn |
lab head | |
Zheng Fang | |
contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
contact email | zhengfang@dicp.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |