PXD045625 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of aal1 long non-coding RNA physically interacting proteins |
Description | Genomes pervasively produce long non-coding RNAs (lncRNAs) of largely unknown functions. Some of these lncRNAs have been implicated in roles related to ageing and associated diseases. Here we characterize aal1 (ageing-associated lncRNA 1) which is induced in non-dividing, ageing cells of fission yeast. Deletion of aal1 shortens the chronological lifespan of non-dividing cells, while ectopic overexpression of aal1 from a plasmid prolongs their lifespan, indicating that this lncRNA acts in trans. We find that aal1 genetically interacts with coding genes functioning in processes related to protein translation, and aal1 overexpression leads to repression of ribosomal protein genes and inhibition of cell growth. The aal1 RNA localizes to the cytoplasm and associates with ribosomes. Notably, aal1 deletion or overexpression is sufficient to increase or decrease the cellular ribosome content, respectively. We identify the mRNA rpl1901, encoding a ribosomal protein, as a binding target of aal1. The expression of rpl1901 is moderately repressed by aal1, and such moderate repression is critical and sufficient to extend the chronological lifespan. Remarkably, expression of the yeast aal1 lncRNA in the fly gut triggers a significant extension of the lifespan in flies. Based on these findings, we propose that the aal1 RNA can reduce the ribosome content by decreasing the levels of the Rpl1901 ribosomal protein, thus attenuating protein translation and promoting longevity. Although the aal1 lncRNA itself is not conserved, its effects in the fly raise the possibility that other organisms feature related mechanisms involving conserved ribosome-associated processes to control ageing. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:16:05.222.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shajahan Anver |
SpeciesList | scientific name: Saccharomyces cerevisiae/Schizosaccharomyces pombe fusant; NCBI TaxID: 589904; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q-Tof Ultima |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-24 12:56:25 | ID requested | |
⏵ 1 | 2024-10-08 11:16:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ribosomes,Fission yeast, Chronological Lifespan |
Contact List
Jürg Bähler |
contact affiliation | Professor, Department of Genetics Evolution and Environment, University College London, UK |
contact email | j.bahler@ucl.ac.uk |
lab head | |
Shajahan Anver |
contact affiliation | University College London |
contact email | s.anver@ucl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045625
- Label: PRIDE project
- Name: Identification of aal1 long non-coding RNA physically interacting proteins