PXD045620

PXD045620 is an original dataset announced via ProteomeXchange.

Title	Development of nucleus-targeted synthetic histone photoaffinity probes
Description	Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding fundamental epigenetic regulatory pathways. Current peptide- or protein-based histone photoaffinity probes have greatly expanded the ability to probe the epigenetic interactome, but in situ profiling in the context of native cells (live cells without genetic manipulations) remains challenging. Here, we developed a nucleus-targeted histone photoaffinity probe with a modular design that integrates a cell-permeable and nuclear localization peptide modules into an hPTM peptide equipped with a photoreactive moiety for profiling the hPTM-mediated interactome in native cells. This probe not only enables the probing of epigenetic interactomes in hard-to-transfect macrophage RAW264.7 cells, but also offers opportunities to characterize the interactome of newly emerging hPTM, such as lactylation, thus expanding the toolbox for profiling of hPTM-mediated PPIs in live cells.
HostingRepository	PRIDE
AnnounceDate	2024-11-21
AnnouncementXML	Submission_2024-11-21_05:12:33.260.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Fan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-23 23:16:05	ID requested
⏵ 1	2024-11-21 05:12:33	announced

Dataset with its publication pending

submitter keyword: nucleus-targeted histone photoaffinity probe, lactylation,the epigenetic interactome, hard-to-transfect cells, native cells

Yi-Ming Li
contact affiliation	School of Food and Biological Engineering, Hefei University of Technology
contact email	ymli@hfut.edu.cn
lab head
Jian Fan
contact affiliation	18255156119
contact email	fanxy@mail.ustc.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD045620

PRIDE project URI

• PRIDE
  1. PXD045620
     1. Label: PRIDE project
     2. Name: Development of nucleus-targeted synthetic histone photoaffinity probes