PXD045614

PXD045614 is an original dataset announced via ProteomeXchange.

Title	Single-cell proteomics analysis of melatonin-treated and MG132-treated in vitro post-ovulatory aged oocytes
Description	Once ovulated, the oocyte has to be fertilized in a short time window, or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell proteomic and phosphoproteomic analysis of fresh, POA, melatonin-treated and MG132-treated POA oocytes.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:52:28.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yueshuai Guo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-23 08:22:31	ID requested
⏵ 1	2025-05-06 11:52:28	announced

Guo Y, Gao M, Liu X, Zhang H, Wang Y, Yan T, Wang B, Han X, Qi Y, Zhu H, Situ C, Li Y, Guo X, Vitro Post-Ovulatory-Aged Oocytes Revealed Aging-Dependent Protein Degradation. Mol Cell Proteomics, 24(1):100882(2025) [pubmed]

10.1016/j.mcpro.2024.100882;

submitter keyword: Melatonin, Oocyte, Mouse, Proteomics, Post-ovulation aging,Single cell proteomics, MG132, Phosphorylation

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University
contact email	guo_xuejiang@njmu.edu.cn
lab head
Yueshuai Guo
contact affiliation	Nanjing Medical University
contact email	gys_706@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045614

PRIDE project URI

• PRIDE
  1. PXD045614
     1. Label: PRIDE project
     2. Name: Single-cell proteomics analysis of melatonin-treated and MG132-treated in vitro post-ovulatory aged oocytes