PXD045607

PXD045607 is an original dataset announced via ProteomeXchange.

Title	Massive intein content in Anaeramoeba reveals new aspects of intein mobility in eukaryotes
Description	Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryote described previously. The Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes and viruses. Our data also suggest that viruses have contributed to the spread of inteins in Anaeramoeba and the colonization of new alleles. The persistence of Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2023-11-30
AnnouncementXML	Submission_2023-11-30_08:15:45.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jon Jerlström Hultqvist
SpeciesList	scientific name: Anaeramoeba flamelloides; NCBI TaxID: 1746091;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 15:45:36	ID requested
⏵ 1	2023-11-30 08:15:46	announced

10.1073/PNAS.2306381120;

submitter keyword: LC-MS/MS, intein, protist, Anaeramoeba

Jon Jerlström
contact affiliation	Microbiology and Immunology, Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Sweden
contact email	jon.jerlstrom.hultqvist@icm.uu.se
lab head
Jon Jerlström Hultqvist
contact affiliation	Department of Cell and Molecular Biology, Uppsala University
contact email	jon.jerlstrom.hultqvist@icm.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045607
     1. Label: PRIDE project
     2. Name: Massive intein content in Anaeramoeba reveals new aspects of intein mobility in eukaryotes