PXD045606

PXD045606 is an original dataset announced via ProteomeXchange.

Title	Aedes aegypti mosquito eggshell proteins in response to RNAi-EOF1
Description	Aedes aegypti mosquito ovarian follicles develop synchronously after taking a blood meal. A single layer of follicular epithelial cells surrounding the oocyte is responsible for secreting a majority of eggshell structural components between 18 and 54 hours post blood meal. We previously identified a protein called eggshell organizing factor 1 (EOF1). When we knocked down EOF1 with RNA interference in female adult mosquitoes, they laid eggs that were not properly melanized, and fragile eggs did not contain viable embryos. Here, we aimed to determine putative downstream eggshell proteins in RNAi-EOF1 female mosquitoes. Our eggshell proteomics identified 220 proteins, suggesting that mosquito extracellular eggshells are composed of a complex mixture of proteins. These proteins are involved in the formation of an intact eggshell that protects the embryonic development and larva from the environment for long periods of time.
HostingRepository	PRIDE
AnnounceDate	2023-09-29
AnnouncementXML	Submission_2023-09-29_15:37:19.861.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045606
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	JunIsoe
SpeciesList	scientific name: Aedes aegypti aegypti; NCBI TaxID: 1424507;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 15:40:21	ID requested
⏵ 1	2023-09-29 15:37:20	announced

10.6019/PXD045606;

submitter keyword: RNA interference, eggshell, melanization,mosquito, reproduction

JunIsoe
contact affiliation	University of Arizona
contact email	jisoe@ag.arizona.edu
lab head
JunIsoe
contact affiliation	The University of Arizona
contact email	jtisoe@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045606

PRIDE project URI

• PRIDE
  1. PXD045606
     1. Label: PRIDE project
     2. Name: Aedes aegypti mosquito eggshell proteins in response to RNAi-EOF1