PXD045600

PXD045600 is an original dataset announced via ProteomeXchange.

Title	Limnospira indica PCC8005 in simulated microgravity
Description	Regenerative life support systems for space crews recycle organic and inorganic waste into water, food and oxygen using different organisms. For instance, the European Space Agency's MELiSSA uses Limnospira indica PCC8005 for air revitalisation and food production. Before use in space, the components' compatibility with reduced gravity must be tested. This innovative study introduces a novel ground analog designed specifically for microgravity experiments involving cyanobacteria, employing a cutting-edge random positioning machine (RPM). Limnospira indica PCC8005 was shown to grow slower under simulated microgravity and whole proteome analysis revealed a downregulation of e.g. ribosomal proteins, glutamine synthase and nitrate uptake transporters while an upregulation was found for gas vesicle proteins, carboxysome proteins and phycobiliproteins. All together our results suggested that L. indica experienced carbon limitation when cultivated in simulated microgravity conditions.
HostingRepository	PRIDE
AnnounceDate	2024-12-19
AnnouncementXML	Submission_2024-12-19_02:09:42.354.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045600
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felice Mastroleo
SpeciesList	scientific name: Limnospira indica PCC 8005; NCBI TaxID: 376219;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 09:04:27	ID requested
⏵ 1	2024-12-19 02:09:42	announced

10.6019/PXD045600;

10.1038/S41526-024-00436-X;

submitter keyword: LC-MSMS, proteomics,cyanobacteria, random positioning machine, arthrospira

Natalie Leys
contact affiliation	Head of the Microbiology unit, SCK-CEN,Belgium
contact email	natalie.leys@sckcen.be
lab head
Felice Mastroleo
contact affiliation	SCK CEN
contact email	felice.mastroleo@sckcen.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD045600

PRIDE project URI

• PRIDE
  1. PXD045600
     1. Label: PRIDE project
     2. Name: Limnospira indica PCC8005 in simulated microgravity