PXD045597

PXD045597 is an original dataset announced via ProteomeXchange.

Title	Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells
Description	Protein O-linked mannose (O-Man) glycosylation is an evolutionary conserved post-translational modification, whose biosynthesis is initiated by three non-redundant enzyme families, POMT1/POMT2, TMTC1-4 and TMEM260. In this study, we applied a targeted workflow for membrane glycoproteomics to five human cell lines and to a panel of genetically engineered cells with individual and combinatorial knock-out of O-Man glycosyltransferase genes to extensively map substrates, specificities, and crosstalk of O-Man enzymes. Our quantitative glycoproteomics results demonstrate new protein targets for the POMT1/POMT2 pathway and show that TMTC1-4 and TMEM260 widely target distinct Ig-like protein domains of plasma membrane proteins. This new evidence adds further knowledge on the emerging concept of domain-specific O-Man glycosylation and establishes a platform for functional studies of O-Man glycosylated adhesion molecules and receptors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:44:14.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adnan Halim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	C-glycosylated residue; O-glycosylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 07:12:57	ID requested
1	2024-06-10 03:27:15	announced
⏵ 2	2024-10-22 06:44:15	announced	2024-10-22: Updated project metadata.

10.1016/j.mcpro.2024.100796;

Povolo L, Tian W, Vakhrushev SY, Halim A, Global View of Domain-Specific O-Linked Mannose Glycosylation in Glycoengineered Cells. Mol Cell Proteomics, 23(7):100796(2024) [pubmed]

submitter keyword: LC-MSMS, O-Man, glycoproteomics, PTM, glycobiology,O-mannose

Adnan Halim
contact affiliation	Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
contact email	halim@sund.ku.dk
lab head
Adnan Halim
contact affiliation	University of Copenhagen
contact email	halim@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045597

PRIDE project URI

• PRIDE
  1. PXD045597
     1. Label: PRIDE project
     2. Name: Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells