PXD045589

PXD045589 is an original dataset announced via ProteomeXchange.

Title	Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification
Description	In the present study, we investigated whether sweat-derived extracellular vesicles (EVs) can be used as a source of potential protein biomarkers of human and bacterial origin. We compared protein composition of total sweat, EV-enriched sweat (after ExoEasy) and sweat samples collected with the use of alginate skin patches. We identified 1209 unique human proteins in EV-enriched sweat, of which approximately 20% were present in every individual sample investigated. Besides human proteins, sweat EV samples contained 1594 proteins of bacterial origin. Bacterial protein profiles in EV-enriched sweat were characterized by high interindividual variability, that reflected differences in total sweat composition. Alginate-based sweat patch accumulated about 30% of human proteins from those identified in sweat-derived EVs, but only 5% proteins of bacterial origin. In conclusion, we showed that sweat-derived EVs provide a rich source of potential biomarkers of human and bacterial origin and that commercially available alginate skin patches could be used for collection of human sweat EVs with very little impact of bacterial vesicles.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:25:52.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anatoliy Samoylenko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 04:19:20	ID requested
1	2024-01-26 07:15:23	announced
⏵ 2	2024-10-22 06:25:54	announced	2024-10-22: Updated project metadata.

Zhyvolozhnyi A, Samoylenko A, Bart G, Kaisanlahti A, Hekkala J, Makieieva O, Pratiwi F, Miinalainen I, Kaakinen M, Bergman U, Singh P, Nurmi T, Khosrowbadi E, Abdelrady E, Kellokumpu S, Kosamo S, Reunanen J, R, ö, ning J, Hiltunen J, Vainio SJ, Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification. Nanotheranostics, 8(1):48-63(2024) [pubmed]

10.7150/ntno.87822;

submitter keyword: sweat, human,Extracellular vesicles, bacteria, alginate

Seppo Vainio
contact affiliation	Faculty of Biochemistry and Molecular Medicine FIN-90014 Oulu University FINLAND
contact email	seppo.vainio@oulu.fi
lab head
Anatoliy Samoylenko
contact affiliation	University of Oulu
contact email	anatoliy.samoylenko@oulu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045589

PRIDE project URI

• PRIDE
  1. PXD045589
     1. Label: PRIDE project
     2. Name: Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification