PXD045589 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification |
Description | In the present study, we investigated whether sweat-derived extracellular vesicles (EVs) can be used as a source of potential protein biomarkers of human and bacterial origin. We compared protein composition of total sweat, EV-enriched sweat (after ExoEasy) and sweat samples collected with the use of alginate skin patches. We identified 1209 unique human proteins in EV-enriched sweat, of which approximately 20% were present in every individual sample investigated. Besides human proteins, sweat EV samples contained 1594 proteins of bacterial origin. Bacterial protein profiles in EV-enriched sweat were characterized by high interindividual variability, that reflected differences in total sweat composition. Alginate-based sweat patch accumulated about 30% of human proteins from those identified in sweat-derived EVs, but only 5% proteins of bacterial origin. In conclusion, we showed that sweat-derived EVs provide a rich source of potential biomarkers of human and bacterial origin and that commercially available alginate skin patches could be used for collection of human sweat EVs with very little impact of bacterial vesicles. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:25:52.849.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anatoliy Samoylenko |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-22 04:19:20 | ID requested | |
1 | 2024-01-26 07:15:23 | announced | |
⏵ 2 | 2024-10-22 06:25:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhyvolozhnyi A, Samoylenko A, Bart G, Kaisanlahti A, Hekkala J, Makieieva O, Pratiwi F, Miinalainen I, Kaakinen M, Bergman U, Singh P, Nurmi T, Khosrowbadi E, Abdelrady E, Kellokumpu S, Kosamo S, Reunanen J, R, รถ, ning J, Hiltunen J, Vainio SJ, Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification. Nanotheranostics, 8(1):48-63(2024) [pubmed] |
10.7150/ntno.87822; |
Keyword List
submitter keyword: sweat, human,Extracellular vesicles, bacteria, alginate |
Contact List
Seppo Vainio |
contact affiliation | Faculty of Biochemistry and Molecular Medicine FIN-90014 Oulu University FINLAND |
contact email | seppo.vainio@oulu.fi |
lab head | |
Anatoliy Samoylenko |
contact affiliation | University of Oulu |
contact email | anatoliy.samoylenko@oulu.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045589
- Label: PRIDE project
- Name: Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification