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PXD045589

PXD045589 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification
DescriptionIn the present study, we investigated whether sweat-derived extracellular vesicles (EVs) can be used as a source of potential protein biomarkers of human and bacterial origin. We compared protein composition of total sweat, EV-enriched sweat (after ExoEasy) and sweat samples collected with the use of alginate skin patches. We identified 1209 unique human proteins in EV-enriched sweat, of which approximately 20% were present in every individual sample investigated. Besides human proteins, sweat EV samples contained 1594 proteins of bacterial origin. Bacterial protein profiles in EV-enriched sweat were characterized by high interindividual variability, that reflected differences in total sweat composition. Alginate-based sweat patch accumulated about 30% of human proteins from those identified in sweat-derived EVs, but only 5% proteins of bacterial origin. In conclusion, we showed that sweat-derived EVs provide a rich source of potential biomarkers of human and bacterial origin and that commercially available alginate skin patches could be used for collection of human sweat EVs with very little impact of bacterial vesicles.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:25:52.849.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnatoliy Samoylenko
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-22 04:19:20ID requested
12024-01-26 07:15:23announced
22024-10-22 06:25:54announced2024-10-22: Updated project metadata.
Publication List
Zhyvolozhnyi A, Samoylenko A, Bart G, Kaisanlahti A, Hekkala J, Makieieva O, Pratiwi F, Miinalainen I, Kaakinen M, Bergman U, Singh P, Nurmi T, Khosrowbadi E, Abdelrady E, Kellokumpu S, Kosamo S, Reunanen J, R, รถ, ning J, Hiltunen J, Vainio SJ, Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification. Nanotheranostics, 8(1):48-63(2024) [pubmed]
10.7150/ntno.87822;
Keyword List
submitter keyword: sweat, human,Extracellular vesicles, bacteria, alginate
Contact List
Seppo Vainio
contact affiliationFaculty of Biochemistry and Molecular Medicine FIN-90014 Oulu University FINLAND
contact emailseppo.vainio@oulu.fi
lab head
Anatoliy Samoylenko
contact affiliationUniversity of Oulu
contact emailanatoliy.samoylenko@oulu.fi
dataset submitter
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Dataset FTP location
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