PXD045587

PXD045587 is an original dataset announced via ProteomeXchange.

Title	The proteome of dinoflagellate symbionts (Durusdinium trenchii) during symbiosis establishment in a model cnidarian
Description	Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Durusdinium trenchii data; Breviolum minutum data are uploaded in a separate entry with identical parameters.
HostingRepository	PRIDE
AnnounceDate	2024-01-18
AnnouncementXML	Submission_2024-01-18_03:40:50.422.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045587
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Durusdinium trenchii; NCBI TaxID: 1381693;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 03:35:16	ID requested
⏵ 1	2024-01-18 03:40:50	announced

10.6019/PXD045587;

submitter keyword: Durusdinium, symbiosis,Dinoflagellate, Breviolum, symbiont, Exaiptasia

Simon K
contact affiliation	School of Biological Sciences, Victoria University of Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045587

PRIDE project URI

• PRIDE
  1. PXD045587
     1. Label: PRIDE project
     2. Name: The proteome of dinoflagellate symbionts (Durusdinium trenchii) during symbiosis establishment in a model cnidarian