PXD045581

PXD045581 is an original dataset announced via ProteomeXchange.

Title	Characterization of RNAseT2 interactome in C2C12 cells
Description	Muscle stem cells (MuSCs) fuse to form multinucleated cells to repair skeletal muscle after injury. While adult MuSCs express a cell autonomous myogenesis program, in vivo investigations demonstrated that the MuSC environment is required for efficient adult myogenesis and muscle regeneration. Particularly, anti-inflammatory/restorative macrophages stimulate MuSC differentiation and fusion, although the molecular mechanisms involved are still largely unknown. Here, we show that restorative macrophages secrete ribonuclease T2 (RNAseT2) which specifically stimulates MuSC fusion. Co-immuprecipitation coupled to mass spectrometry-based proteomics identified Ste20-like kinase (SLK) as binding partner of RNAseT2. SLK triggered the phosphorylation-mediated activation of N-WASP, allowing actin remodeling at the time of MuSC fusion.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:14:23.156.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD045581
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 02:12:42	ID requested
⏵ 1	2026-06-14 16:14:23	announced

10.6019/PXD045581;

Weiss-Gayet M, Juban G, Le Moal E, Moretta A, Farnetani C, Gobet C, Guillemaud J, Le Bihan MC, Shoseyov O, Adrait A, Ternka K, Boespflug-Tanguy O, Kettwig M, Cout, é Y, Mounier R, Acquati F, Knight RD, Chazaud B, Restorative macrophage-derived RNAseT2 stimulates muscle stem cell fusion via an SLK/N-WASP/actin bundling dependent axis. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-71990-z;

submitter keyword: co-immunoprecipitation,Skeletal mucle injury, Muscle stem cells, ribonuclease T2, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD045581

PRIDE project URI

• PRIDE
  1. PXD045581
     1. Label: PRIDE project
     2. Name: Characterization of RNAseT2 interactome in C2C12 cells