PXD045580

PXD045580 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification and functional characterization of calcineurin substrates in tomato Fusarium wilt fungus
Description	Tomato is a worldwide economic crop, and Fusarium wilt is one of the important tomato diseases which is caused by Fusarium oxysporum f. sp. lycopersici (Fol). The typical symptoms are yellowing and wilting on leaves and browning vascular tissues. Subsequently, it may cause tomato to die or no fruit production. Calcineurin is a heterodimeric phosphatase which can be activated by Ca2+/calmodulin and can dephosphorylate downstream substrates to regulate stress survival, growth, sexual differentiation, and virulence. Calcineurin substrates have been studied in Saccharomyces cerevisiae by phosphoproteomics and quantitative mass spectrometry. To date, there is no research related to proteome analysis of calcineurin downstream substrates in plant pathogens. In our previous study, it revealed that calcineurin is responsible for hyphal growth, conidia formation, chlamydospore formation, and virulence in Fol. In our phosphoproteomics analysis, we compared the protein abundance of wild -type and Δcna1 strains with calcium or non-calcium induced condition. Next, we used Scanprosite and Uniport to predict conserved docking and dephosphorylated sites within those proteins by the abundance more than 2-fold distribution. Finally, we obtained 59 putative calcineurin substrates. According to GO enrichment analysis, these substrates included transcription factors, transmembrane transporters, protein serine/threonine kinase activator, and some uncharacterized proteins. We propose that these putative substrates may play important roles to control physiological function and virulence in Fol.
HostingRepository	jPOST
AnnounceDate	2024-09-22
AnnouncementXML	Submission_2024-09-21_08:00:05.190.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu-Yu Lin
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 00:26:52	ID requested
⏵ 1	2024-09-21 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: tomato Fusarium wilt, Fusarium oxysporum f. sp. lycopersici, calcineurin, phosphoproteomics

submitter keyword: tomato Fusarium wilt, Fusarium oxysporum f. sp. lycopersici, calcineurin, phosphoproteomics

Contact List

Ying-Lien Chen
lab head
Yu-Yu Lin
contact affiliation	Department of Plant Pathology and Microbiology, National Taiwan University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002329/