PXD045579

PXD045579 is an original dataset announced via ProteomeXchange.

Title	Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain
Description	The glial environment determines the outcome of neurological disease progression, yet much of our knowledge still relies on preclinical animal studies, especially regarding astrocyte heterogeneity. In murine models of traumatic brain injury, beneficial functions of proliferating reactive astrocytes on disease outcome have been unraveled, but little is known if and when they are present in human brain pathology. Here, we examined a broad spectrum of pathologies with and without intracerebral hemorrhage and found a striking correlation between lesions involving blood-brain barrier rupture and astrocyte proliferation that was further corroborated in an assay probing for neural stem cell potential. Most importantly, proteomic analysis unraveled a crucial signaling pathway regulating this astrocyte plasticity with GALECTIN3 as a novel marker for proliferating astrocytes and the GALECTIN3-binding protein LGALS3BP as a functional hub mediating astrocyte proliferation and neurosphere formation. Taken together, this work identifies a therapeutically relevant astrocyte response and their molecular regulators in different pathologies affecting the human cerebral cortex.
HostingRepository	PRIDE
AnnounceDate	2023-12-08
AnnouncementXML	Submission_2023-12-08_02:03:57.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefanie Hauck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 00:16:08	ID requested
⏵ 1	2023-12-08 02:03:57	announced

10.1038/S41591-023-02644-6;

submitter keyword: neural stem cells,human brain injury, astrocyte proliferation, Galectin-LGALS3BP signalling, neurological diseases, CSF

Magdalena Götz
contact affiliation	Chair of Physiological Genomics, Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Germany
contact email	magdalena.goetz@helmholtz-muenchen.de
lab head
Stefanie Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Zentrum München
contact email	hauck@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045579
     1. Label: PRIDE project
     2. Name: Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain