PXD045549

PXD045549 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI)
Description	Nicotiana benthamiana tissue expressing either AvrRpm1-3xFlag, StrepII-3xHA-MAMI, or co-expressing AvrRpm1-3xFlag and StrepII-3xHA-MAMI were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:04:07.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Wirthmueller
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-21 02:02:26	ID requested
1	2023-09-28 00:01:12	announced
2	2023-11-14 08:48:07	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:04:08	announced	2024-10-22: Updated project metadata.

10.1101/2023.09.25.558804;

submitter keyword: Arabidopsis thaliana, type III effector, Pseudomonas syringae, plant pathogen,ADP-ribosylation, Nicotiana bethamiana

Lennart Wirthmueller
contact affiliation	Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
contact email	lennart.wirthmueller@ipb-halle.de
lab head
Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry Dept. Biochemistry of Plant Interactions Weinberg 3 06120 Halle (Saale)
contact email	lennart.wirthmueller@ipb-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045549

PRIDE project URI

• PRIDE
  1. PXD045549
     1. Label: PRIDE project
     2. Name: Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI)