PXD045541

PXD045541 is an original dataset announced via ProteomeXchange.

Title	β-hydroxybutyrate facilitates mitochondrial-derived vesicle biogenesis and improves mitochondrial quality control
Description	Mitochondrial dynamics and metabolites reciprocally influence each other. Mitochondrial-derived vesicles (MDVs) transport damaged mitochondrial components to lysosomes or the extracellular space, and they are an emerging mechanism that regulates mitochondrial quality. While many metabolites are known to modulate mitochondrial dynamics, it is largely unclear whether they are involved in MDVs generation. Here, we discovered that the major component of ketone body, β-hydroxybutyrate (BHB), improved mitochondrial quality control by facilitating the biogenesis of MDVs. Mechanistically, BHB supplementation drove lysine β-hydroxybutyrylation (Kbhb) of SNX9, a key regulator of MDV biogenesis, at three specific lysine residues. Kbhb increased SNX9 interaction with inner mitochondrial membrane (IMM)/matrix proteins and promoted the formation of IMM/matrix MDVs. Lastly, we found that SNX9 Kbhb not only played a critical role in maintaining mitochondrial homeostasis in cells, but also protected mice from alcohol-induced liver injury. Altogether, our research uncovers that metabolites influence the formation of MDVs by directly engaging in post-translational modifications of key protein machineries and establishes a new framework for understanding how metabolites regulate mitochondrial quality
HostingRepository	iProX
AnnounceDate	2023-09-21
AnnouncementXML	Submission_2023-09-20_23:22:30.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diudiu Tufei
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 23:22:04	ID requested
⏵ 1	2023-09-20 23:22:30	announced

Dataset with its publication pending

submitter keyword: β-hydroxybutyrate, mitochondrial-derived vesicle, mitochondrial quality control

Lunzhi Dai
contact affiliation	Sichuan University
contact email	lunzhi.dai@scu.edu.cn
lab head
Diudiu Tufei
contact affiliation	Sichuan University
contact email	niqiu.er@163.com
dataset submitter

iProX dataset URI