PXD045527

PXD045527 is an original dataset announced via ProteomeXchange.

Title	Drosophila melanogaster hemolymph gelatinase
Description	The innate immune response of insects relies on several humoral and cellular mechanisms that require the activation of circulating proteases in the hemolymph to be functional. Here, we analyzed the gelatinase and caseinase activities of Drosophila larval hemolymph under normal and pathogenic conditions (bacterial lipopolysaccharides or endoparasitoid Leptopilina boulardi) using in gel zymography. Gelatinase activity was more intense than caseinase activity and qualitative and quantitative variations were observed between D. melanogaster strains and Drosophila species. Mass spectrometry identified a large number of serine proteases in gel bands equivalent to the major gelatinase and caseinase bands and of these, the most abundant and redundant were Tequila and members of the Jonah and Trypsin protease families. However, hemolymph from Tequila null mutant larvae showed no obvious changes in zymographic bands. Nor did we observe any significant changes in hemolymph gelatinases activity 24 h after injection of bacterial lipopolysaccharides or after oviposition by endoparasitoid wasps. These data confirmed that many serine proteases are present in Drosophila larval hemolymph but those with gelatinase and caseinase activity may not change drastically during the immune response.
HostingRepository	PRIDE
AnnounceDate	2024-04-29
AnnouncementXML	Submission_2024-04-29_02:46:03.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maya BELGHAZI
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 08:48:44	ID requested
⏵ 1	2024-04-29 02:46:04	announced

10.3390/insects15040234;

Gatti JL, Lemauf S, Belghazi M, Arthaud L, Poiri, é M, In Drosophila Hemolymph, Serine Proteases Are the Major Gelatinases and Caseinases. Insects, 15(4):(2024) [pubmed]

submitter keyword: Gelatinase, Immunity, Caseinase, in gel zymography,Drosophila, Hemolymph, Proteases

Jean-Luc GATTI
contact affiliation	Evolution and Specificity of Multitrophic Interactions (ESIM) Sophia Agrobiotech Institute (ISA) INRAE, Université Côte d’Azur, CNRS 06903 Sophia Antipolis Cedex France
contact email	jean-luc.gatti@inrae.fr
lab head
Maya BELGHAZI
contact affiliation	CNRS
contact email	maya.belghazi@univ-amu.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD045527

PRIDE project URI

• PRIDE
  1. PXD045527
     1. Label: PRIDE project
     2. Name: Drosophila melanogaster hemolymph gelatinase