PXD045502

PXD045502 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Reveal the Role of Matrine in Lipid Metabolism
Description	Hyperlipidemia (HLP) can lead to cardiovascular diseases (CVDs) and other urgent diseases, such as myocardial infarction (MI) and stroke. So, early therapy for HLP is necessary. As existing drugs to treat HLP could not achieve the ideal effect for some patients, we have to find new drugs for HLP treatment. More and more evidence has shown that matrine has the function in treating HLP, but the mechanism of matrine in treating HLP is still unknown. In this study, we investigated the mechanism of matrine in treating HLP by performing quantitative proteomics. We discovered that matrine can upregulate the gene transcription and expression of LDLR and improve the uptake of LDL in HepG2 cells. Results also showed that the upregulated LDLR mediated by matrine plays an important role in reversing disordered lipid metabolism in vivo. As the disordered lipid metabolism is related to the development of lung cancer and matrine has been reported to have the function of treating lung cancer, we explored whether the regulation of lipid metabolism mediated by metrine has effect on the development of lung cancer. Through performing quantitative proteomics in A549, we noticed that the dynamic proteins regulated by matrine mainly enriched in the pathways related to lipid metabolism. And the expression of LDLR was also promoted by matrine in A549 cells. As studies have found that upregulated and prolonged uptake of LDL is detrimental to breast cancer cells, we speculated matrine exerts its anti-lung cancer function through promoting the expression of LDLR.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:23.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 19:27:04	ID requested
1	2024-06-22 08:25:11	announced
⏵ 2	2024-10-22 06:46:24	announced	2024-10-22: Updated project metadata.

10.1021/acsomega.3c09983;

Feng H, Liu G, Li L, Ren X, Jiang Y, Hou W, Liu R, Liu K, Liu H, Huang H, Quantitative Proteomics Reveal the Role of Matrine in Regulating Lipid Metabolism. ACS Omega, 9(23):24308-24320(2024) [pubmed]

submitter keyword: Matrine, Lipid Metabolism, Hyperlipidemia, Proteomics

He Huang
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	hhuang@simm.ac.cn
lab head
He Huang
contact affiliation	Shanghai Institute of Materia Medica
contact email	hhuang.nu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045502

PRIDE project URI

• PRIDE
  1. PXD045502
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Reveal the Role of Matrine in Lipid Metabolism