PXD045497

PXD045497 is an original dataset announced via ProteomeXchange.

Title	Methionine Alkylation as an Approach to Quantify Methionine Oxidation Using Mass Spectrometry
Description	Post-translational oxidation of methionine residues can destabilize proteins or modify their functions. Although levels of methionine oxidation can provide important information regarding the structural integrity and regulation of proteins, their quantitation is often challenging as analytical procedures in and of themselves can artifactually oxidize methionines. Here, we develop a mass spectrometry-based method called Methionine Oxidation by Blocking with Alkylation (MObBa) that accurately quantifies methionine oxidation by selectively alkylating and blocking unoxidized methionines. Thus, alkylated methionines can be used as a stable proxy for unoxidized methionines. Using proof of concept experiments, we demonstrate that MObBa can be used to accurately quantify methionine oxidation levels within individual synthetic peptides and on proteome-wide scales. MObBa may provide a straightforward experimental strategy for mass spectrometric quantitation of methionine oxidation.
HostingRepository	PRIDE
AnnounceDate	2024-02-08
AnnouncementXML	Submission_2024-02-08_07:02:18.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margaret Hoare
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 12:21:43	ID requested
⏵ 1	2024-02-08 07:02:19	announced

10.1021/JASMS.3C00337;

submitter keyword: Methionine oxidation

Methionine alkylation

Proteomics, Mass spectrometry (MS)

Sina Ghaemmaghami
contact affiliation	Department of Biology, University of Rochester, NY, 14627, USA University of Rochester Mass Spectrometry Resource Laboratory, NY, 14627, USA
contact email	sina.ghaemmaghami@rochester.edu
lab head
Margaret Hoare
contact affiliation	University of Rochester
contact email	mhoare@u.rochester.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD045497

PRIDE project URI

• PRIDE
  1. PXD045497
     1. Label: PRIDE project
     2. Name: Methionine Alkylation as an Approach to Quantify Methionine Oxidation Using Mass Spectrometry