PXD045496

PXD045496 is an original dataset announced via ProteomeXchange.

Title	Experience-induced remodeling of the hippocampal post-synaptic proteome and phosphoproteome
Description	The postsynaptic density (PSD) of excitatory synapses contains a highly organized protein network with thousands of proteins and is key node in the regulation of synaptic plasticity. To gain new mechanistic insight into experience-induced changes in the PSD, we examined the global dynamics of the hippocampal PSD proteome and phosphoproteome in mice following 4 different types of experience. Mice were trained using an inhibitory avoidance (IA) task and hippocampal PSD fractions were isolated from individual mice to investigate molecular mechanisms underlying experience-dependent remodeling of synapses. We developed a new strategy to identify and quantify the relatively low level of site-specific phosphorylation of PSD proteome from the hippocampus, by using a modified iTRAQ-based TiSH protocol. In the PSD, we identified 3,938 proteins and 2,761 phosphoproteins in the sequential strategy covering a total of 4,968 unique protein groups (at least 2 peptides including an unique peptide). On the phosphoproteins, we identified a total of 6,188 unambiguous phosphosites (75% site localization probability)
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:43.530.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045496
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Taewook Kang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 11:59:44	ID requested
1	2024-01-26 10:58:28	announced
⏵ 2	2024-10-22 06:27:45	announced	2024-10-22: Updated project metadata.

10.6019/PXD045496;

Heo S, Kang T, Bygrave AM, Larsen MR, Huganir RL, Experience-Induced Remodeling of the Hippocampal Post-synaptic Proteome and Phosphoproteome. Mol Cell Proteomics, 22(11):100661(2023) [pubmed]

10.1016/j.mcpro.2023.100661;

submitter keyword: proteomics,Mouse, phosphoproteomics

Taewook Kang
contact affiliation	University of Southern Denmark
contact email	systemkang@gmail.com
lab head
Taewook Kang
contact affiliation	University of Southern Denmark
contact email	taewookk@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045496

PRIDE project URI

• PRIDE
  1. PXD045496
     1. Label: PRIDE project
     2. Name: Experience-induced remodeling of the hippocampal post-synaptic proteome and phosphoproteome