PXD045482

PXD045482 is an original dataset announced via ProteomeXchange.

Title	Proximal proteins of the type I interferon signaling cascade
Description	Deciphering the intricate dynamic events governing type I interferon (IFN) signaling is critical to unravel key regulatory mechanisms in host antiviral defense. Here, we leveraged TurboID-based proximity labeling coupled with affinity purification-mass spectrometry to comprehensively map temporal changes to the proximal human proteomes of all seven canonical type I IFN signaling cascade members following IFN stimulation. This established a network of 108 proteins in close proximity to the core members IFNAR1, IFNAR2, JAK1, TYK2, STAT1, STAT2, and IRF9, and validated several known protein assemblies, while also revealing novel, transient associations between key signaling molecules.
HostingRepository	PRIDE
AnnounceDate	2024-05-28
AnnouncementXML	Submission_2024-05-28_05:30:31.188.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samira Schiefer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 03:28:02	ID requested
⏵ 1	2024-05-28 05:30:32	announced

10.1038/s41467-024-48800-5;

Schiefer S, Hale BG, Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by PJA2. Nat Commun, 15(1):4484(2024) [pubmed]

submitter keyword: IFN, TurboID, interferon, proximity labeling, JAK/STAT

Ben Hale
contact affiliation	Institute of Medical Virology, University of Zurich, Switzerland
contact email	hale.ben@virology.uzh.ch
lab head
Samira Schiefer
contact affiliation	Institute of Medical Virology, University of Zurich
contact email	schiefer.samira@virology.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045482

PRIDE project URI

• PRIDE
  1. PXD045482
     1. Label: PRIDE project
     2. Name: Proximal proteins of the type I interferon signaling cascade