PXD045481

PXD045481 is an original dataset announced via ProteomeXchange.

Title	Impact of media supplementation on a transcriptional activity of porcine embryos produced in vitro
Description	The production of embryos in vitro caused a revolution in the field of assisted reproduction technologies, which currently allows the manipulation and selection of embryos before implantation. One of the key aspects of this process is the maturation of oocytes in a controlled in vitro environment. In this article, we focused on the impact of precisely chemically modified FLI maturation medium and its potential in improving the efficiency of in vitro production of porcine embryos. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro maturation, in vitro embryo production, EGA detection, transcriptomic profile and proteomic analysis, which was confirmed by the positive activation of the embryonal genome in the 4blastoembry stage of parthenogenetically activated embryos.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:16:44.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Morovic
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 02:23:31	ID requested
⏵ 1	2024-10-08 11:16:44	announced

Dataset with its publication pending

submitter keyword: DMEM, maturation medium, oocyte, FLI, porcine

Martin Morovic
contact affiliation	Department of Zoology and Anthropology, Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra
contact email	mmorovic@ukf.sk
lab head
Martin Morovic
contact affiliation	Constantine teh Philosopher University in Nitra Faculty of Natural Sciences and Informatics Department of Zoology and Anthropology
contact email	mmorovic@ukf.sk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045481

PRIDE project URI

• PRIDE
  1. PXD045481
     1. Label: PRIDE project
     2. Name: Impact of media supplementation on a transcriptional activity of porcine embryos produced in vitro