<<< Full experiment listing

PXD045473

PXD045473 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRNF185 control of COL3A1 expression limits prostate cancer migration and metastatic potential.
DescriptionRNF185 is a RING finger domain-containing ubiquitin ligase implicated in ER-associated degradation. Prostate tumor patient data analysis revealed a negative correlation between RNF185 expression and prostate cancer progression and metastasis. Likewise, several prostate cancer cell lines exhibited greater migration and invasion capabilities in culture upon RNF185 depletion. Subcutaneous inoculation of mouse prostate cancer MPC3 cells stably expressing shRNA against RNF185 into mice resulted in larger tumors and more frequent lung metastases.RNA-sequencing and Ingenuity Pathway Analysis identified wound healing and cellular movement among the most significant pathways upregulated in RNF185-depleted, compared to control prostate cancer cells. Gene Set Enrichment Analyses performed in samples from patients harboring low RNF185 expression and in RNF185-depleted lines confirmed the deregulation of genes implicated in EMT. Among those, COL3A1 was identified as the primary mediator of RNF185’s ability to impact migration phenotypes. Correspondingly, enhanced migration and metastasis of RNF185 KD prostate cancer cells were attenuated upon co-inhibition of COL3A1. Our results identify RNF185 as a gatekeeper of prostate cancer metastasis, partly via its control of COL3A1 availability.
HostingRepositoryPRIDE
AnnounceDate2024-08-09
AnnouncementXMLSubmission_2024-08-09_05:13:46.361.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Van Espen
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcarbamoylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-18 15:13:28ID requested
12024-08-09 05:13:47announced
Publication List
Van Espen B, Oo HZ, Collins C, Fazli L, Molinolo A, Yip K, Murad R, Gleave M, Ronai ZA, RNF185 Control of COL3A1 Expression Limits Prostate Cancer Migration and Metastatic Potential. Mol Cancer Res, 22(1):41-54(2024) [pubmed]
10.1158/1541-7786.mcr-23-0512;
Keyword List
submitter keyword: ubiquitin ligase
RNF185
prostate adenocarcinoma
metastasis
epithelial-mesenchymal transition (EMT)
collagen
Contact List
Ze'ev Ronai
contact affiliationDirector, NCI-Designated Cancer Center Professor, Cancer Metabolism and Microenvironment Program Sanford Burnham Prebys Medical Discovery Institute 10901 North Torrey Pines Road La Jolla, CA 92037
contact emailronai@sbpdiscovery.org
lab head
Benjamin Van Espen
contact affiliationSanford Burnham Prebys Medical Discovery Institute
contact emailbvanespen@sbpdiscovery.org
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD045473
PRIDE project URI
Repository Record List
[ + ]