PXD045433

PXD045433 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Extending the coverage of Lys-C/trypsin-based bottom-up proteomics using cysteine S-aminoethylation
Description	In this study, S-aminoethylation of Cys (AE-Cys) was carried out with 2-bromoethylamine to form pseudo-Lys, which was then digested with Lys-C or trypsin to improve proteome coverage in bottom-up proteomics. A model study with bovine serum albumin showed that the C-terminal side of Cys was successfully cleaved by AE-Cys followed by digestion with Lys-C. The frequency of side reactions to amino acids other than Cys was not as high as that of carbamidomethylation of Cys with iodoacetamide. Proteomic analysis of A549 cell extracts using AE-Cys identified more protein groups, especially membrane proteins, in a data-dependent acquisition mode. In addition, label-free quantification of mouse non-small cell lung cancer (NSCLC) tissue in data-independent acquisition mode showed improved NSCLC pathway coverage and higher reproducibility with AE-Cys. Furthermore, we successfully identified peptides containing the [T790M] mutation site of EGFR by AE-Cys, which has not been reported by conventional bottom-up methods despite its importance as one of the most important lung cancer-related mutation sites.
HostingRepository	jPOST
AnnounceDate	2023-09-27
AnnouncementXML	Submission_2023-09-26_08:00:21.970.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryota Tomioka
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; S-carboxamidomethyl-L-cysteine
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-18 00:09:42	ID requested
⏵ 1	2023-09-26 08:00:22	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: S-aminoethylation, alkyration, membrane proteins

submitter keyword: S-aminoethylation, alkyration, membrane proteins

Contact List

Yasushi Ishihama
lab head
Ryota Tomioka
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002323/