PXD045429
PXD045429 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tumor mitochondrial DNA and antigen-loaded nanoparticles encapsulated with dendritic cell derived microvesicles elicits long lasting anti-tumor immune responses by activating the cGAS-STING pathway |
Description | Scarcity of dendritic cells (DCs) impact lung and pancreatic tumor immune status despite limited clinical benefits from autologous tumor lysate-pulsed DC vaccination. To tackle this issue, we developed a DC-based immunotherapy utilizing cationic nanoparticles (cNPs) loaded with tumor or organoid lysate encapsulated within DC-derived microvesicles (cNPcancer cell@MVDC). Remarkably, cNPcancer cell@MVDC treatment converted immune cold tumors into a hot microenvironment, leading to increased migratory DC population, reduced tumor growth and improved survival in orthotopic animal models compared to mature DC treatment. In vivo tracking experiments demonstrated superior accumulation of cNPcancer cell@MVDC in tumors and draining lymph nodes (dLNs) compared to mature DCs, promoting DC migration to dLNs and activating CD8+ T cells. Clinically, the accurate prediction of tumor immune status, immunotherapy response and patient prognosis relies on migratory DC infiltration rather than CD8+ T cells. Mechanistically, tumor lysate pulsed-cNPs enriched mitochondrial DNA, which exhibited a stronger binding affinity with cGAS compared to nuclear DNA, resulting in enhanced cGAS-STING-mediated DC activation. This immunotherapy elicits durable anti-tumor immune responses, even in immune-deserted tumor environments. |
HostingRepository | iProX |
AnnounceDate | 2023-09-16 |
AnnouncementXML | Submission_2024-05-21_18:43:23.392.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xue Jiang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-09-17 20:55:59 | ID requested | |
⏵ 1 | 2024-05-21 18:43:23 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Pancreatic ductal adenocarcinomas, Dendritic cell, Microvesicles, cGAS, mitochondrial DNA |
Contact List
Pingpui Wong | |
---|---|
contact affiliation | Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University |
contact email | huangbp3@mail.sysu.edu.cn |
lab head | |
Xue Jiang | |
contact affiliation | Sun Yat-sen Memorial Hospital, Sun Yat-sen University |
contact email | 915143522@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |