PXD045420
PXD045420 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ArreSTick Motif is Responsible for GPCR-B-Arrestin Binding Stability and Extends Phosphorylation-Dependent B-arrestin Interactions to Non-Receptor Proteins |
| Description | The binding and function of B-arrestins are regulated by specific phosphorylation motifs present in G protein-coupled receptors (GPCRs). However, the exact arrangement of phosphorylated amino acids responsible for establishing a stable interaction remains unclear. To investigate this pattern, we employed a 1D sequence convolution model trained on a dataset of GPCRs that have established B-arrestin binding properties. This approach allowed us to identify the amino acid pattern required for GPCRs to form stable interactions with B-arrestins. This motif was named 'arreSTick.' Our data show that the model predicts the strength of the coupling between GPCRs and B-arrestins with high accuracy, as well as the specific location of the interaction within the receptor sequence. Furthermore, we show that the arreSTick pattern is not limited to GPCRs, and is also present in numerous non-receptor proteins. Using a proximity biotinylation assay and mass spectrometry analysis, we demonstrate that the arreSTick motif controls the interaction between numerous non-receptor proteins and B-arrestins. For example, the HIV-1 Tat Specific Factor 1 (HTSF1 or HTATSF1), a nuclear transcription factor, contains the arreSTick pattern, and our data show that its subcellular localization is influenced by its coupling to B-arrestin2. Our findings unveil a broader regulatory role for B-arrestins in phosphorylation-dependent interactions, extending beyond GPCRs to encompass non-receptor proteins as well. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-05-10 |
| AnnouncementXML | Submission_2024-05-10_06:03:07.809.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Laszlo Drahos |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | maXis II |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-15 10:25:30 | ID requested | |
| ⏵ 1 | 2024-05-10 06:03:08 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: arrestins |
Contact List
| Gabor Turu | |
|---|---|
| contact affiliation | Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences, Research Centre for Natural Sciences, Magyar Tudosok krt. 2., H-1117, Budapest, Hungary |
| contact email | turu.gabor@ttk.hu |
| lab head | |
| Laszlo Drahos | |
| contact affiliation | RCNS |
| contact email | drahos.laszlo@ttk.hu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092868/ |
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