PXD045382 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sse1, Hsp110 chaperone of yeast, controls the cellular fate during Endoplasmic Reticulum-stress |
Description | Sse1, yeast cytosolic Hsp110 chaperone, is a wellknown Nucleotide Exchange Factor (NEF), a protein-disaggregase and a Chaperone linked to Protein Synthesis (CLIPS). Here we demonstrate SSE1’s genetic interaction with IRE1 and HAC1, the Endoplasmic Reticulum-Unfolded Protein Response (ER-UPR) sensors. sse1Δ strain exhibits an ER-UPR signalling-dependent resistance to tunicamycin-induced ER stress. Importantly, ER-stress-responsive reorganization of translating ribosomes from polysomes to monosomes is inefficient in SSE1 deleted strain leading to uninterrupted protein translation and starkly different ER-UPR kinetics. sse1Δ exhibits faster ER-UPR induction and quicker reversal to basal state compared to wildtype (WT) cells. Interestingly, ER-stress mediated yeast cell division arrest is escaped in sse1Δ strain during long term tunicamycin stress indicating important role of this chaperone in controlling cell division during ER stress. Furthermore, sse1Δ strain shows significantly higher cell viability in comparison to WT yeast, following short-term as well as long-term tunicamycin stress. In summary, we show that cytosolic chaperone Sse1 genetically interacts with ER-UPR pathway, controls the kinetics of ER-UPR, stress-induced cell division arrest and cell viability during global ER stress by tunicamycin. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-19 |
AnnouncementXML | Submission_2024-04-18_22:40:17.367.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mainak Pratim Jha |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-14 04:05:31 | ID requested | |
⏵ 1 | 2024-04-18 22:40:18 | announced | |
Publication List
10.1093/g3journal/jkae075; |
Jha MP, Kumar V, Ghosh A, Mapa K, Sse1, Hsp110 chaperone of yeast, controls the cellular fate during endoplasmic reticulum stress. G3 (Bethesda), 14(6):(2024) [pubmed] |
Keyword List
submitter keyword: cell cycle arrest, Heat Shock Protein 110, Endoplasmic Reticulum Unfolded Protein Response,Molecular Chaperone, Tunicamycin, Protein Homeostasis, Heat Shock Protein 70 |
Contact List
Koyeli Mapa |
contact affiliation | Protein Homeostasis Laboratory, Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence, Delhi-NCR, Greater Noida, Gautam Buddha Nagar, Uttar Pradesh 201314, India. |
contact email | koyeli.mapa@snu.edu.in |
lab head | |
Mainak Pratim Jha |
contact affiliation | PhD Scholar, Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence (University) |
contact email | mj216@snu.edu.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045382
- Label: PRIDE project
- Name: Sse1, Hsp110 chaperone of yeast, controls the cellular fate during Endoplasmic Reticulum-stress