PXD045359
PXD045359 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A systematic and validated Fe(II)-EDTA method of hydroxyl radical generation for oxidative protein footprinting |
| Description | Correlating the structure and dynamics of proteins with biological function is critical to understanding normal and dysfunctional cellular mechanisms. We describe a quantitative method of hydroxyl radical generation via Fe(II)-EDTA catalyzed Fenton chemistry that provides easy access to oxidative protein footprinting using equipment commonly found in research and process control laboratories. Robust and reproducible dose-dependent oxidation of protein samples is observed and quantitated by mass spectrometry with as fine as single residue resolution. An oxidation analysis of lysozyme provides a readily accessible benchmark for our method. The efficacy of our oxidation method is demonstrated through mapping the interface of a RAS monobody complex, the surface of the NIST mAb, and the interface between PRC2 complex components. These studies are executed using standard laboratory tools and a few pennies of reagents; the mass spectrometry analysis can be streamlined to map protein structure with single amino acid residue resolution. All mass spectrometry raw files for this study are included here. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-09-13 |
| AnnouncementXML | Submission_2023-09-13_10:54:26.760.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Beatrix Ueberheide |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | LTQ Orbitrap Elite; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-13 10:04:54 | ID requested | |
| ⏵ 1 | 2023-09-13 10:54:27 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Fenton reaction, oxidative footprinting |
Contact List
| Beatrix Ueberheide | |
|---|---|
| contact affiliation | NYU Langone Health |
| contact email | beatrix.ueberheide@nyulangone.org |
| lab head | |
| Beatrix Ueberheide | |
| contact affiliation | NYU School of Medicine |
| contact email | beatrixueberheide@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092853/ |
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