PXD045348

PXD045348 is an original dataset announced via ProteomeXchange.

Title	Proteomic dataset of exosomes derived from fetal mesenchymal stromal cells
Description	The raw files of the proteomics dataset are N=18 and a brief description of the files is shown below: AF1 raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 1 AF2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 2 AFA1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 3 AFA2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 4 AFB1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 5 AFB2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 6 HL1A.raw: exosomes derived from Hepatocyte like cells 1 HL1B.raw: exosomes derived from Hepatocyte like cells 2 HL2A.raw: exosomes derived from Hepatocyte like cells 3 HL2B.raw: exosomes derived from Hepatocyte like cells 4 HL3A.raw: exosomes derived from Hepatocyte like cells 5 HL3B.raw: exosomes derived from Hepatocyte like cells 6 HPLA1.raw: exosomes derived from Hepatic Progenitor-like cells 1 HPLA2.raw: exosomes derived from Hepatic Progenitor-like cells 2 HPLB1.raw: exosomes derived from Hepatic Progenitor-like cells 3 HPLB2.raw: exosomes derived from Hepatic Progenitor-like cells 4 HPLA.raw: exosomes derived from Hepatic Progenitor-like cells 5 HPLB.raw: exosomes derived from Hepatic Progenitor-like cells 6 Raw files analysis was performed with Proteome Discoverer 1.4 (Thermo) software package, using the Sequest search engine and the Uniprot human (Homo sapiens) reviewed database, downloaded on December 15, 2017, including 20,243 entries. The search was performed using carbamidomethylation of cysteine as static and oxidation of methionine as dynamic modifications. Two missed cleavage sites, a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.05 Da were allowed. False discovery rate (FDR) validation was based on q value: target FDR : 0.05. Label free quantification was performed by utilizing the precursor ion area values exported from the total ion chromatogram as defined by the Proteome Discoverer v. 1.4.0.288 (Thermo Scientific). Output files from Proteome Discoverer were processed with R programming language for statistical computing (version 4.0.3). The following comparisons were made: AF vs HL, AF vs HPL and HL vs HPL. The msf files are N=18 and have the same name as the raw files above.
HostingRepository	MassIVE
AnnounceDate	2023-12-01
AnnouncementXML	Submission_2023-12-01_07:24:27.611.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manousos Makridakis
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification; unknown modification
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-13 04:28:34	ID requested
⏵ 1	2023-12-01 07:24:28	announced

no publication

submitter keyword: mesenchymal stromal cells, acute hepatic failure, differentiation, EVs, secretome, proteomics, LC-MS/MS, MFGE-8

Maria (Maya) Roubelakis
contact affiliation	Laboratory of Biology, Medical School of Athens National and Kapodistrian University of Athens and Center of Basic Research Biomedical Research Foundation of the Academy of Athens
contact email	roubel@med.uoa.gr
lab head
Manousos Makridakis
contact affiliation	Biomedical Research Foundations, Academy of Athens
contact email	mmakrid@bioacademy.gr
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092848/